ARG56612
anti-NANOG antibody
anti-NANOG antibody for ELISA,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Cow,Dog,Pig
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes NANOG |
|---|---|
| 反应物种 | Hu, Ms, Cow, Dog, Pig |
| 应用 | ELISA, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | NANOG |
| 抗原物种 | Human |
| 抗原 | E.coli derived Recombinant Human Nanog. (SVDPACPQSL PCFEASDCKE SSPMPVICGP EENYPSLQMS SAEMPHTETV SPLPSSMDLL IQDSPDSSTS PKGKQPTSAE NSVAKKEDKV PVKKQKTRTV FSSTQLCVLN DRFQRQKYLS LQQMQELSNI LNLSYKQVKT WFQNQRMKSK RWQKNNWPKN SNGVTQKASA PTYPSLYSSY HQGCLVNPTG NLPMWSNQTW NNSTWSNQTQ NIQSWSNHSW NTQTWCTQSW NNQAWNSPFY NCGEESLQSC MQFQPNSPAS DLEAALEAAG EGLNVIQQTT RYFSTPQTMD LFLNYSMNMQ PEDV) |
| 偶联标记 | Un-conjugated |
| 別名 | Homeobox transcription factor Nanog; Homeobox protein NANOG; hNanog |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | PBS (pH 7.2) |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | NANOG |
| 全名 | Nanog homeobox |
| 背景介绍 | The protein encoded by this gene is a DNA binding homeobox transcription factor involved in embryonic stem (ES) cell proliferation, renewal, and pluripotency. The encoded protein can block ES cell differentiation and can also autorepress its own expression in differentiating cells. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2015] |
| 生物功能 | Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. Acts as a transcriptional activator or repressor. Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3'. Able to autorepress its expression in differentiating (ES) cells: binds to its own promoter following interaction with ZNF281/ZFP281, leading to recruitment of the NuRD complex and subsequent repression of expression. When overexpressed, promotes cells to enter into S phase and proliferation. [UniProt] |
| 预测分子量 | 35 kDa |
检测图片 (5) Click the Picture to Zoom In
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ARG56612 anti-NANOG antibody IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded sections of Human prostate malignant adenocarcinoma. The recommended ARG56612 anti-NANOG antibody concentration is 0.5 µg/ml with an overnight incubation at 4°C. An alkaline phosphatase-labeled polymer detection system was used with a non-alcohol soluble red chromogen. Antigen Retrieval: Boil tissue section in Sodium Citrate buffer (pH 6.0) followed by cooling at RT for 20 min.
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ARG56612 anti-NANOG antibody WB image
Western blot: 250 - 0.24 ng of Human Nanog stained with ARG56612 anti-NANOG antibody, under reducing conditions.
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ARG56612 anti-NANOG antibody IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded sections of Human prostate malignant adenocarcinoma. The recommended ARG56612 anti-NANOG antibody concentration is 0.5 µg/ml with an overnight incubation at 4°C. An alkaline phosphatase-labeled polymer detection system was used with a non-alcohol soluble red chromogen. Antigen Retrieval: Boil tissue section in Sodium Citrate buffer (pH 6.0) followed by cooling at RT for 20 min.
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ARG56612 anti-NANOG antibody WB image
Western blot: 250 - 0.24 ng of Human Nanog stained with ARG56612 anti-NANOG antibody, under non-reducing conditions.
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ARG56612 anti-NANOG antibody standard curve image
Sandwich ELISA: ARG56612 anti-NANOG antibody as a capture antibody at 0.5 - 2.0 µg/ml combined with ARG56727 anti-NANOG antibody (Biotin) as a detection antibody. Results of a typical standard run with optical density.
