ARG56959
anti-NANOG antibody [5A10]
anti-NANOG antibody [5A10] for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
参考文献1
概述
| 产品描述 | Mouse Monoclonal antibody [5A10] recognizes NANOG |
|---|---|
| 反应物种 | Hu, Ms |
| 应用 | FACS, IHC-P, WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | 5A10 |
| 同位型 | IgG2a, kappa |
| 靶点名称 | NANOG |
| 抗原物种 | Human |
| 抗原 | Recombinant fragment around aa. 1-154 of Human Nanog. |
| 偶联标记 | Un-conjugated |
| 別名 | Homeobox transcription factor Nanog; Homeobox protein NANOG; hNanog |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|---|---|---|---|
| 应用说明 | IHC-P: Antigen Retrieval: Boil tissue section in 0.1M Sodium citrate buffer (pH 6.0) for 20 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein G. |
| 缓冲液 | PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 10% Glycerol |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | NANOG |
| 全名 | Nanog homeobox |
| 背景介绍 | The protein encoded by this gene is a DNA binding homeobox transcription factor involved in embryonic stem (ES) cell proliferation, renewal, and pluripotency. The encoded protein can block ES cell differentiation and can also autorepress its own expression in differentiating cells. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2015] |
| 生物功能 | Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. Acts as a transcriptional activator or repressor. Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3'. Able to autorepress its expression in differentiating (ES) cells: binds to its own promoter following interaction with ZNF281/ZFP281, leading to recruitment of the NuRD complex and subsequent repression of expression. When overexpressed, promotes cells to enter into S phase and proliferation. [UniProt] |
| 预测分子量 | 35 kDa |
检测图片 (3) Click the Picture to Zoom In
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ARG56959 anti-NANOG antibody [5A10] FACS image
Flow Cytometry: Hep3B cell line stained with ARG56959 anti-NANOG antibody [5A10] at 2-5 µg for 1x10^6 cells. Secondary antibody: Goat anti-Mouse IgG Alexa fluor 488 conjugate.
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ARG56959 anti-NANOG antibody [5A10] IHC-P image
Immunohistochemistry: Paraffin embedded sections of Human seminoma tissue stained with ARG56959 anti-NANOG antibody [5A10] at 1:50 for 2 hours at RT. Antigen Retrieval: Boil tissue section in 0.1M Sodium citrate buffer (pH 6.0) for 20 min.
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ARG56959 anti-NANOG antibody [5A10] WB image
Western blot: 35 µg of NIH3T3 cell lysate stained with ARG56959 anti-NANOG antibody [5A10] at 1:500.
文献引用
Stem cell characteristics promote aggressiveness of diffuse large B-cell lymphoma.
WB / Human
