ARG59180
anti-NAE1 / APPBP1 antibody
anti-NAE1 / APPBP1 antibody for IHC-Formalin-fixed paraffin-embedded sections and Human
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes NAE1 / APPBP1 |
|---|---|
| 反应物种 | Hu |
| 预测物种 | Ms, Rat |
| 应用 | IHC-P |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | NAE1 / APPBP1 |
| 抗原物种 | Human |
| 抗原 | KLH-conjugated synthetic peptide within aa. 430-459 of Human NAE1 / APPBP1. |
| 偶联标记 | Un-conjugated |
| 別名 | APP-BP1; APPBP1; A-116A10.1; Amyloid protein-binding protein 1; HPP1; Amyloid beta precursor protein-binding protein 1, 59 kDa; NEDD8-activating enzyme E1 regulatory subunit; ula-1; Proto-oncogene protein 1 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein G. |
| 缓冲液 | PBS and 0.09% (W/V) Sodium azide. |
| 抗菌剂 | 0.09% (W/V) Sodium azide. |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q13564 Human NEDD8-activating enzyme E1 regulatory subunit |
|---|---|
| 基因名称 | NAE1 |
| 全名 | NEDD8 activating enzyme E1 subunit 1 |
| 背景介绍 | The protein encoded by this gene binds to the beta-amyloid precursor protein. Beta-amyloid precursor protein is a cell surface protein with signal-transducing properties, and it is thought to play a role in the pathogenesis of Alzheimer's disease. In addition, the encoded protein can form a heterodimer with UBE1C and bind and activate NEDD8, a ubiquitin-like protein. This protein is required for cell cycle progression through the S/M checkpoint. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| 生物功能 | Regulatory subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBE2M. Necessary for cell cycle progression through the S-M checkpoint. Overexpression of NAE1 causes apoptosis through deregulation of NEDD8 conjugation. [UniProt] |
| 细胞定位 | Cell membrane. Note=Colocalizes with APP in lipid rafts. [UniProt] |
| 预测分子量 | 60 kDa |
| 翻译后修饰 | Ubiquitinated by TRIP12, leading to its degradation by the proteasome. [UniProt] |
检测图片 (1) Click the Picture to Zoom In
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ARG59180 anti-NAE1 / APPBP1 antibody IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Human breast carcinoma stained with ARG59180 anti-NAE1 / APPBP1 antibody.
