ARG43963

anti-NADK2 antibody

anti-NADK2 antibody for ELISA,Flow cytometry,ICC/IF,Western blot and Human,Mouse,Rat

概述

产品描述 Rabbit Polyclonal antibody recognizes NADK2
反应物种 Hu, Ms, Rat
应用 ELISA, FACS, ICC/IF, WB
宿主 Rabbit
克隆 Polyclonal
同位型 IgG
靶点名称 NADK2
抗原物种 Human
抗原 Human NADK2 recombinant protein
表达系统 E.coli
偶联标记 Un-conjugated
Protein Full name NAD kinase 2, mitochondrial
別名 NADK2; NAD Kinase 2, Mitochondrial; MNADK; C5orf33; NADKD1; NAD Kinase Domain-Containing Protein 1, Mitochondrial; Mitochondrial NAD Kinase; FLJ30596; Chromosome 5 Open Reading Frame 33; NAD Kinase Domain Containing 1; EC 2.7.1.23; DECRD

应用说明

应用建议
应用 推荐稀释比
ELISA0.1-0.5 µg/ml
FACS1-3 µg /1x10^6 cells
ICC/IF5 µg/ml
WB0.25-0.5 µg/ml
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式 Liquid
纯化 Affinity purified with Immunogen.
缓冲液 0.9% NaCl, 0.2% Na2HPO4 and 4% Trehalose.
稳定剂 4% Trehalose
浓度 0.5 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 133686 Human NADK2

GeneID: 365699 Rat NADK2

GeneID: 68646 Mouse NADK2

基因名称 NADK2
全名 NAD Kinase 2, Mitochondrial
背景介绍 This gene encodes a mitochondrial kinase that catalyzes the phosphorylation of NAD to yield NADP. Mutations in this gene result in 2,4-dienoyl-CoA reductase deficiency. Alternative splicing results in multiple transcript variants.
生物功能 Mitochondrial NAD+ kinase that phosphorylates NAD+ to yield NADP+. Can use both ATP or inorganic polyphosphate as the phosphoryl donor. Also has weak NADH kinase activity in vitro; however NADH kinase activity is much weaker than the NAD+ kinase activity and may not be relevant in vivo.
细胞定位 Mitochondrion
预测分子量 49 kDa
翻译后修饰 Acetylation, Phosphoprotein

检测图片 (5) Click the Picture to Zoom In

  • ARG43963 anti-NADK2 antibody ICC/IF image

    Immunofluorescence: A549 cells stained withARG43963 anti-NADK2 antibody at 5 μg/ml dilution.

  • ARG43963 anti-NADK2 antibody WB image

    Western blot: HepG2 and Hela stained with ARG43963 anti-NADK2 antibody at 0.5 μg/mL dilution.

  • ARG43963 anti-NADK2 antibody FACS image

    Flow Cytometry: HepG2 cells stained with ARG43963 anti-NADK2 antibody (blue) at 1 μg/1x10^6 cells dilution.

  • ARG43963 anti-NADK2 antibody WB image

    Western blot: Rat brain stained with ARG43963 anti-NADK2 antibody at 0.5 μg/mL dilution.

  • ARG43963 anti-NADK2 antibody WB image

    Western blot: Mouse brain tissue lysates and Mouse thymus stained with ARG43963 anti-NADK2 antibody at 0.5 μg/mL dilution.