ARG42576
anti-NAA60 / NAT15 antibody
anti-NAA60 / NAT15 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes NAA60 / NAT15 |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | NAA60 / NAT15 |
| 抗原物种 | Human |
| 抗原 | Recombinant fusion protein corresponding to aa. 1-242 of Human NAA60 / NAT15 (NP_079121.1). |
| 偶联标记 | Un-conjugated |
| 別名 | EC 2.3.1.48; N-alpha-acetyltransferase 60; Histone acetyltransferase type B protein 4; N-acetyltransferase 15; NatF catalytic subunit; HAT4; EC 2.3.1.88; NAT15 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| 阳性对照 | Mouse brain | ||||||
| 实际分子量 | ~ 32 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified. |
| 缓冲液 | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | NAA60 |
| 全名 | N(alpha)-acetyltransferase 60, NatF catalytic subunit |
| 背景介绍 | This gene encodes an enzyme that localizes to the Golgi apparatus, where it transfers an acetyl group to the N-terminus of free proteins. This enzyme acts on histones, and its activity is important for chromatin assembly and chromosome integrity. Alternative splicing and the use of alternative promoters results in multiple transcript variants. The upstream promoter is located in a differentially methylated region (DMR) and undergoes imprinting; transcript variants originating from this position are expressed from the maternal allele. [provided by RefSeq, Nov 2015] |
| 生物功能 | N-alpha-acetyltransferase that specifically mediates the acetylation of N-terminal residues of the transmembrane proteins, with a strong preference for N-termini facing the cytosol (PubMed:25732826). Displays N-terminal acetyltransferase activity towards a range of N-terminal sequences including those starting with Met-Lys, Met-Val, Met-Ala and Met-Met (PubMed:21750686, PubMed:25732826, PubMed:27550639, PubMed:27320834). Required for normal chromosomal segregation during anaphase (PubMed:21750686). May also show histone acetyltransferase activity; such results are however unclear in vivo and would require additional experimental evidences (PubMed:21981917). [UniProt] |
| 细胞定位 | Golgi apparatus membrane; Peripheral membrane protein; Cytoplasmic side. Note=Probably forms a intramembrane hairpin-like structure in the membrane. [UniProt] |
| 预测分子量 | 27 kDa |
| 翻译后修饰 | Acetylated: autoacetylation is required for optimal acetyltransferase activity. [UniProt] |
检测图片 (2) Click the Picture to Zoom In
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ARG42576 anti-NAA60 / NAT15 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human liver cancer tissue stained with ARG42576 anti-NAA60 / NAT15 antibody at 1:200 dilution.
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ARG42576 anti-NAA60 / NAT15 antibody WB image
Western blot: 25 µg of Mouse brain lysate stained with ARG42576 anti-NAA60 / NAT15 antibody at 1:1000 dilution.
