ARG42554
anti-NAA10 / ARD1A antibody
anti-NAA10 / ARD1A antibody for ICC/IF,Western blot and Human
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes NAA10 / ARD1A |
|---|---|
| 反应物种 | Hu |
| 应用 | ICC/IF, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | NAA10 / ARD1A |
| 抗原物种 | Human |
| 抗原 | KLH-conjugated synthetic peptide between aa. 135-167 of Human NAA10 / ARD1A. |
| 偶联标记 | Un-conjugated |
| 別名 | N-alpha-acetyltransferase 10; ARD1P; N-terminal acetyltransferase complex ARD1 subunit homolog A; DXS707; EC 2.3.1.88; ARD1; NatA catalytic subunit Naa10; EC 2.3.1.-; ARD1A; TE2; MCOPS1; NATD |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| 阳性对照 | HeLa | ||||||
| 实际分子量 | ~ 30 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein A and immunogen peptide. |
| 缓冲液 | PBS and 0.09% (W/V) Sodium azide. |
| 抗菌剂 | 0.09% (W/V) Sodium azide |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | NAA10 |
| 全名 | N(alpha)-acetyltransferase 10, NatA catalytic subunit |
| 背景介绍 | N-alpha-acetylation is among the most common post-translational protein modifications in eukaryotic cells. This process involves the transfer of an acetyl group from acetyl-coenzyme A to the alpha-amino group on a nascent polypeptide and is essential for normal cell function. This gene encodes an N-terminal acetyltransferase that functions as the catalytic subunit of the major amino-terminal acetyltransferase A complex. Mutations in this gene are the cause of Ogden syndrome. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2012] |
| 生物功能 | Catalytic subunit of the N-terminal acetyltransferase A (NatA) complex which displays alpha (N-terminal) acetyltransferase activity (PubMed:15496142, PubMed:19826488, PubMed:19420222, PubMed:20145209, PubMed:27708256, PubMed:25489052, PubMed:29754825). Acetylates amino termini that are devoid of initiator methionine (PubMed:19420222). The alpha (N-terminal) acetyltransferase activity may be important for vascular, hematopoietic and neuronal growth and development. Without NAA15, displays epsilon (internal) acetyltransferase activity towards HIF1A, thereby promoting its degradation (PubMed:12464182). Represses MYLK kinase activity by acetylation, and thus represses tumor cell migration (PubMed:19826488). Acetylates, and stabilizes TSC2, thereby repressing mTOR activity and suppressing cancer development (PubMed:20145209). Acetylates HSPA1A and HSPA1B at 'Lys-77' which enhances its chaperone activity and leads to preferential binding to co-chaperone HOPX (PubMed:27708256). Acetylates HIST1H4A (PubMed:29754825). Acts as a negative regulator of sister chromatid cohesion during mitosis (PubMed:27422821). [UniProt] |
| 细胞定位 | Cytoplasm. Nucleus. Note=Also present in the free cytosolic and cytoskeleton-bound polysomes. [UniProt] |
| 预测分子量 | 26 kDa |
| 翻译后修饰 | Cleaved by caspases during apoptosis. Phosphorylation by IKBKB/IKKB at Ser-209 promotes its proteasome-mediated degradation. Autoacetylated at Lys-136 which stimulates its catalytic activity. [UniProt] |
检测图片 (2) Click the Picture to Zoom In
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ARG42554 anti-NAA10 / ARD1A antibody ICC/IF image
Immunofluorescence: 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized U2OS cells stained with ARG42554 anti-NAA10 / ARD1A antibody (green) at 1:25 dilution. DAPI (blue) for nuclear staining.
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ARG42554 anti-NAA10 / ARD1A antibody WB image
Western blot: 20 µg of HeLa whole cell lysate stained with ARG42554 anti-NAA10 / ARD1A antibody at 1:2000 dilution.
