ARG42902
anti-N4BP1 antibody
anti-N4BP1 antibody for ICC/IF,Western blot and Human,Mouse
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes N4BP1 |
|---|---|
| 反应物种 | Hu, Ms |
| 应用 | ICC/IF, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | N4BP1 |
| 抗原物种 | Mouse |
| 抗原 | Recombinant fusion protein corresponding to aa. 190-590 of Mouse N4BP1 (NP_085040.2). |
| 偶联标记 | Un-conjugated |
| 別名 | N4BP1; NEDD4-binding protein 1 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| 阳性对照 | Jurkat | ||||||
| 实际分子量 | ~ 125 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified. |
| 缓冲液 | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | N4BP1 |
| 全名 | NEDD4 binding protein 1 |
| 生物功能 | Inhibitor of the E3 ubiquitin-protein ligase ITCH. Acts by interacting with the second WW domain of ITCH, leading to compete with ITCH's substrates and impairing ubiquitination of substrates (By similarity). [UniProt] |
| 细胞定位 | Nucleus, nucleolus. Nucleus, PML body. Note=Primarily localizes to the nucleolus. Also localizes to the PML nuclear bodies, when desumoylated (By similarity). [UniProt] |
| 预测分子量 | 99 kDa |
| 翻译后修饰 | Monoubiquitinated by NEDD4. Polyubiquitinated, leading to its degradation by the proteasome. Sumoylated with SUMO1, abrogating polyubiquitination and subsequent degradation. Desumoylated by SENP1, leading to accumulation in PML nuclear bodies (By similarity). [UniProt] |
检测图片 (2) Click the Picture to Zoom In
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ARG42902 anti-N4BP1 antibody ICC/IF image
Immunofluorescence: U2OS cells stained with ARG42902 anti-N4BP1 antibody at 1:100 dilution.
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ARG42902 anti-N4BP1 antibody WB image
Western blot: 25 µg of Jurkat cell lysate stained with ARG42902 anti-N4BP1 antibody at 1:1000 dilution.
