概述

产品描述	Mouse Monoclonal antibody recognizes N-Ras
反应物种	Hu, Rat
应用	WB
宿主	Mouse
克隆	Monoclonal
克隆号	822CT17.5.1
同位型	IgG1
靶点名称	N-Ras
抗原物种	Human
抗原	KLH-conjugated synthetic peptide corresponding to aa. 70-101 of Human N-Ras.
偶联标记	Un-conjugated
別名	GTPase NRas; N-ras; NRAS1; CMNS; Transforming protein N-Ras; NCMS; ALPS4; NS6

应用说明

应用建议	应用 推荐稀释比 WB 1:1000
应用说明	* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
阳性对照	A431

属性

形式	Liquid
纯化	Purification with Protein G.
缓冲液	PBS and 0.09% (W/V) Sodium azide.
抗菌剂	0.09% (W/V) Sodium azide.
存放说明	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项	For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接	GeneID: 24605 Rat NRAS GeneID: 4893 Human NRAS Swiss-port # P01111 Human GTPase NRas Swiss-port # Q04970 Rat GTPase NRas
基因名称	NRAS
全名	neuroblastoma RAS viral (v-ras) oncogene homolog
背景介绍	This is an N-ras oncogene encoding a membrane protein that shuttles between the Golgi apparatus and the plasma membrane. This shuttling is regulated through palmitoylation and depalmitoylation by the ZDHHC9-GOLGA7 complex. The encoded protein, which has intrinsic GTPase activity, is activated by a guanine nucleotide-exchange factor and inactivated by a GTPase activating protein. Mutations in this gene have been associated with somatic rectal cancer, follicular thyroid cancer, autoimmune lymphoproliferative syndrome, Noonan syndrome, and juvenile myelomonocytic leukemia. [provided by RefSeq, Jun 2011]
生物功能	Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. [UniProt]
细胞定位	Cell membrane; Lipid-anchor; Cytoplasmic side. Golgi apparatus membrane; Lipid-anchor. Note=Shuttles between the plasma membrane and the Golgi apparatus
预测分子量	21 kDa
翻译后修饰	Palmitoylated by the ZDHHC9-GOLGA7 complex. A continuous cycle of de- and re-palmitoylation regulates rapid exchange between plasma membrane and Golgi. Acetylation at Lys-104 prevents interaction with guanine nucleotide exchange factors (GEFs).

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