anti-N Cadherin antibody [SQab22260]

anti-N Cadherin antibody [SQab22260] for IHC-Formalin-fixed paraffin-embedded sections and Human

EMT Study antibody; Mesenchymal Markers antibody


产品描述 Recombinant Rabbit Monoclonal antibody [SQab22260] recognizes N Cadherin
反应物种 Hu
应用 IHC-P
宿主 Rabbit
克隆 Monoclonal
克隆号 SQab22260
同位型 IgG
靶点名称 N Cadherin
抗原物种 Human
抗原 Synthetic peptide corresponding to residues within C-Terminal of N-Cadherin.
偶联标记 Un-conjugated
Protein Full name Cadherin-2
別名 Neural cadherin; N-cadherin; CDw325; CDHN; CD antigen CD325; NCAD; Cadherin-2; CD325


应用 推荐稀释比
IHC-P1:100 - 1:200
应用说明 IHC-P: Antigen Retrieval: Heat mediated was performed using Tris/EDTA buffer (pH 9.0). Incubate the samples at RT (18-25°C) for 30 min.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
阳性对照 Human liver tissue


形式 Liquid
纯化 Purification with Protein A.
缓冲液 PBS, 0.01% Sodium azide, 40% Glycerol and 0.05%BSA.
抗菌剂 0.01% Sodium azide
稳定剂 40% Glycerol and 0.05%BSA
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.



GeneID: 1000 Human CDH2

Swiss-port # P19022 Human Cadherin-2

基因名称 CDH2
全名 cadherin 2, type 1, N-cadherin (neuronal)
背景介绍 N Cadherin is a classical cadherin and member of the cadherin superfamily. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein is proteolytically processed to generate a calcium-dependent cell adhesion molecule and glycoprotein. This protein plays a role in the establishment of left-right asymmetry, development of the nervous system and the formation of cartilage and bone. [provided by RefSeq, Nov 2015]
生物功能 N Cadherin is a calcium-dependent cell adhesion protein; preferentially mediates homotypic cell-cell adhesion by dimerization with a CDH2 chain from another cell. Cadherins may thus contribute to the sorting of heterogeneous cell types. Acts as a regulator of neural stem cells quiescence by mediating anchorage of neural stem cells to ependymocytes in the adult subependymal zone: upon cleavage by MMP24, CDH2-mediated anchorage is affected, leading to modulate neural stem cell quiescence. CDH2 may be involved in neuronal recognition mechanism. In hippocampal neurons, may regulate dendritic spine density. [UniProt]
细胞定位 Cell membrane, Single-pass type I membrane protein. [UniProt]
研究领域 EMT Study antibody; Mesenchymal Markers antibody
预测分子量 100 kDa
翻译后修饰 Cleaved by MMP24. Ectodomain cleavage leads to the generation of a soluble 90 kDa amino-terminal soluble fragment and a 45 kDa membrane-bound carboxy-terminal fragment 1 (CTF1), which is further cleaved by gamma-secretase into a 35 kDa. Cleavage in neural stem cells by MMP24 affects CDH2-mediated anchorage of neural stem cells to ependymocytes in the adult subependymal zone, leading to modulate neural stem cell quiescence (By similarity).

May be phosphorylated by OBSCN. [UniProt]

检测图片 (1) Click the Picture to Zoom In

  • ARG66921 anti-N Cadherin antibody [SQab22260] IHC-P image

    Immunohistochemistry: Formalin/PFA-fixed and paraffin-embedded sections of Human liver tissue stained with ARG66921 anti-N Cadherin antibody [SQab22260]. Antigen Retrieval: Heat tissue section in Tris-EDTA buffer (pH 9.0).