anti-N Cadherin antibody [SQab22260]
anti-N Cadherin antibody [SQab22260] for IHC-Formalin-fixed paraffin-embedded sections and Human
EMT Study antibody; Mesenchymal Markers antibody
|产品描述||Recombinant Rabbit Monoclonal antibody [SQab22260] recognizes N Cadherin|
|抗原||Synthetic peptide corresponding to residues within C-Terminal of N-Cadherin.|
|Protein Full name||Cadherin-2|
|別名||Neural cadherin; N-cadherin; CDw325; CDHN; CD antigen CD325; NCAD; Cadherin-2; CD325|
|应用说明||IHC-P: Antigen Retrieval: Heat mediated was performed using Tris/EDTA buffer (pH 9.0). Incubate the samples at RT (18-25°C) for 30 min.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
|阳性对照||Human liver tissue|
|纯化||Purification with Protein A.|
|缓冲液||PBS, 0.01% Sodium azide, 40% Glycerol and 0.05%BSA.|
|抗菌剂||0.01% Sodium azide|
|稳定剂||40% Glycerol and 0.05%BSA|
|存放说明||For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.|
|注意事项||For laboratory research only, not for drug, diagnostic or other use.|
|全名||cadherin 2, type 1, N-cadherin (neuronal)|
|背景介绍||N Cadherin is a classical cadherin and member of the cadherin superfamily. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein is proteolytically processed to generate a calcium-dependent cell adhesion molecule and glycoprotein. This protein plays a role in the establishment of left-right asymmetry, development of the nervous system and the formation of cartilage and bone. [provided by RefSeq, Nov 2015]|
|生物功能||N Cadherin is a calcium-dependent cell adhesion protein; preferentially mediates homotypic cell-cell adhesion by dimerization with a CDH2 chain from another cell. Cadherins may thus contribute to the sorting of heterogeneous cell types. Acts as a regulator of neural stem cells quiescence by mediating anchorage of neural stem cells to ependymocytes in the adult subependymal zone: upon cleavage by MMP24, CDH2-mediated anchorage is affected, leading to modulate neural stem cell quiescence. CDH2 may be involved in neuronal recognition mechanism. In hippocampal neurons, may regulate dendritic spine density. [UniProt]|
|细胞定位||Cell membrane, Single-pass type I membrane protein. [UniProt]|
|研究领域||EMT Study antibody; Mesenchymal Markers antibody|
|翻译后修饰||Cleaved by MMP24. Ectodomain cleavage leads to the generation of a soluble 90 kDa amino-terminal soluble fragment and a 45 kDa membrane-bound carboxy-terminal fragment 1 (CTF1), which is further cleaved by gamma-secretase into a 35 kDa. Cleavage in neural stem cells by MMP24 affects CDH2-mediated anchorage of neural stem cells to ependymocytes in the adult subependymal zone, leading to modulate neural stem cell quiescence (By similarity).
May be phosphorylated by OBSCN. [UniProt]
检测图片 (1) Click the Picture to Zoom In