ARG66921
anti-N Cadherin antibody [SQab22260]
anti-N Cadherin antibody [SQab22260] for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
EMT Study antibody; Mesenchymal Markers antibody

2
概述
产品描述 | Recombinant Rabbit Monoclonal antibody [SQab22260] recognizes N Cadherin |
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反应物种 | Hu, Ms, Rat |
应用 | IHC-P, WB |
宿主 | Rabbit |
克隆 | Monoclonal |
克隆号 | SQab22260 |
同位型 | IgG |
靶点名称 | N Cadherin |
抗原物种 | Human |
抗原 | Synthetic peptide corresponding to residues within C-Terminal of N-Cadherin. |
偶联标记 | Un-conjugated |
Protein Full name | Cadherin-2 |
別名 | Neural cadherin; N-cadherin; CDw325; CDHN; CD antigen CD325; NCAD; Cadherin-2; CD325 |
应用说明
应用建议 |
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应用说明 | IHC-P: Antigen Retrieval: Heat mediated was performed using Tris/EDTA buffer (pH 9.0). Incubate the samples at RT (18-25°C) for 30 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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阳性对照 | Human liver tissue | ||||||
实际分子量 | 140 kDa |
属性
形式 | Liquid |
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纯化 | Purification with Protein A. |
缓冲液 | PBS, 0.01% Sodium azide, 40% Glycerol and 0.05%BSA. |
抗菌剂 | 0.01% Sodium azide |
稳定剂 | 40% Glycerol and 0.05%BSA |
存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
数据库连接 | |
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基因名称 | CDH2 |
全名 | cadherin 2, type 1, N-cadherin (neuronal) |
背景介绍 | N Cadherin is a classical cadherin and member of the cadherin superfamily. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein is proteolytically processed to generate a calcium-dependent cell adhesion molecule and glycoprotein. This protein plays a role in the establishment of left-right asymmetry, development of the nervous system and the formation of cartilage and bone. [provided by RefSeq, Nov 2015] |
生物功能 | N Cadherin is a calcium-dependent cell adhesion protein; preferentially mediates homotypic cell-cell adhesion by dimerization with a CDH2 chain from another cell. Cadherins may thus contribute to the sorting of heterogeneous cell types. Acts as a regulator of neural stem cells quiescence by mediating anchorage of neural stem cells to ependymocytes in the adult subependymal zone: upon cleavage by MMP24, CDH2-mediated anchorage is affected, leading to modulate neural stem cell quiescence. CDH2 may be involved in neuronal recognition mechanism. In hippocampal neurons, may regulate dendritic spine density. [UniProt] |
细胞定位 | Cell membrane, Single-pass type I membrane protein. [UniProt] |
研究领域 | EMT Study antibody; Mesenchymal Markers antibody |
预测分子量 | 100 kDa |
翻译后修饰 | Cleaved by MMP24. Ectodomain cleavage leads to the generation of a soluble 90 kDa amino-terminal soluble fragment and a 45 kDa membrane-bound carboxy-terminal fragment 1 (CTF1), which is further cleaved by gamma-secretase into a 35 kDa. Cleavage in neural stem cells by MMP24 affects CDH2-mediated anchorage of neural stem cells to ependymocytes in the adult subependymal zone, leading to modulate neural stem cell quiescence (By similarity). May be phosphorylated by OBSCN. [UniProt] |
检测图片 (3) Click the Picture to Zoom In
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ARG66921 anti-N Cadherin antibody [SQab22260] IHC-P image
Immunohistochemistry: Formalin/PFA-fixed and paraffin-embedded sections of Human liver tissue stained with ARG66921 anti-N Cadherin antibody [SQab22260]. Antigen Retrieval: Heat tissue section in Tris-EDTA buffer (pH 9.0).
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ARG66921 anti-N Cadherin antibody [SQab22260] WB image (Customer review)
Western blot: Mouse brain stained with ARG66921 anti-N Cadherin antibody [SQab22260] at 1:500 dilution.
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ARG66921 anti-N Cadherin antibody [SQab22260] WB image (Customer review)
Western blot: Rat brain stained with ARG66921 anti-N Cadherin antibody [SQab22260] at 1:500 dilution.
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