ARG23870

anti-N Cadherin antibody

anti-N Cadherin antibody for ELISA,Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Mouse,Rat,Sheep

EMT Study antibody; Mesenchymal Markers antibody
nuts_pic
1
anti-N Cadherin antibody Rabbit Polyclonal antibody recognizes N Cadherin ARG23870 Arigo biolaboratories
5.0 out of 5 based on 1
anti-N Cadherin antibody is rated 5.0 out of 5 by 1.

anti-N Cadherin antibody

概述

产品描述 Rabbit Polyclonal antibody recognizes N Cadherin
反应物种 Hu, Ms, Rat, Sheep
预测物种 Chimp, Chk
应用 ELISA, FACS, ICC/IF, IHC-P, IP, WB
特异性 The antibody detects a band at ~ 130 kDa in western blots of Human endothelial cells and Mouse brain tissue, and does not cross-react with E Cadherin.
宿主 Rabbit
克隆 Polyclonal
同位型 IgG
靶点名称 N Cadherin
抗原物种 Human
抗原 Carrier protein coupled synthetic peptide corresponding to aa. 811-824 of Human N Cadherin.
偶联标记 Un-conjugated
全名 cadherin 2, type 1, N-cadherin (neuronal)
別名 Neural cadherin; N-cadherin; CDw325; CDHN; CD antigen CD325; NCAD; Cadherin-2; CD325

应用说明

应用建议
应用 推荐稀释比
ELISA1:2000
FACS1:100
ICC/IF1:200
IHC-P1 µg/ml
IP1:100
WB1:1000
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
预测分子量 100 kDa
实际分子量 ~ 130 kDa

属性

形式 Liquid
纯化 Affinity purification with immunogen.
缓冲液 PBS, 0.05% Sodium azide, 50% Glycerol and 1 mg/ml BSA.
抗菌剂 0.05% Sodium azide.
稳定剂 50% Glycerol and 1 mg/ml BSA
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 1000 Human CDH2

GeneID: 12558 Mouse CDH2

GeneID: 83501 Rat CDH2

基因名称 CDH2
基因全名 cadherin 2, type 1, N-cadherin (neuronal)
背景介绍 N Cadherin is a classical cadherin and member of the cadherin superfamily. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein is proteolytically processed to generate a calcium-dependent cell adhesion molecule and glycoprotein. This protein plays a role in the establishment of left-right asymmetry, development of the nervous system and the formation of cartilage and bone. [provided by RefSeq, Nov 2015]
生物功能 N Cadherin is a calcium-dependent cell adhesion protein; preferentially mediates homotypic cell-cell adhesion by dimerization with a CDH2 chain from another cell. Cadherins may thus contribute to the sorting of heterogeneous cell types. Acts as a regulator of neural stem cells quiescence by mediating anchorage of neural stem cells to ependymocytes in the adult subependymal zone: upon cleavage by MMP24, CDH2-mediated anchorage is affected, leading to modulate neural stem cell quiescence. CDH2 may be involved in neuronal recognition mechanism. In hippocampal neurons, may regulate dendritic spine density. [UniProt]
细胞定位 Cell membrane; Single-pass type I membrane protein. Cell membrane, sarcolemma. Cell junction. Cell surface. Note=Colocalizes with TMEM65 at the intercalated disk in cardiomyocytes. Colocalizes with OBSCN at the intercalated disk and at sarcolemma in cardiomyocytes. [UniProt]
产品亮点 Related Antibody Duos and Panels:
ARG30320 EMT Marker Antibody Panel
Related products:
N Cadherin antibodies; N Cadherin ELISA Kits; N Cadherin Duos / Panels; Anti-Rabbit IgG secondary antibodies;
Related news:
New EMT antibody panel is released
研究领域 EMT Study antibody; Mesenchymal Markers antibody
翻译后修饰 Cleaved by MMP24. Ectodomain cleavage leads to the generation of a soluble 90 kDa amino-terminal soluble fragment and a 45 kDa membrane-bound carboxy-terminal fragment 1 (CTF1), which is further cleaved by gamma-secretase into a 35 kDa. Cleavage in neural stem cells by MMP24 affects CDH2-mediated anchorage of neural stem cells to ependymocytes in the adult subependymal zone, leading to modulate neural stem cell quiescence (By similarity).

May be phosphorylated by OBSCN. [UniProt]

检测图片 (5) Click the Picture to Zoom In

  • ARG23870 anti-N Cadherin antibody WB image

    Western blot: 20 µg of mouse brain stained with ARG23870 anti-N Cadherin antibody at 1:1000 dilution. (Observed MW: ~130 kDa)

  • ARG23870 anti-N Cadherin antibody ICC/IF image

    Immunofluorescence: MCF7-ADR cells stained with ARG23870 anti-N Cadherin antibody.

  • ARG23870 anti-N Cadherin antibody IHC-P image

    Immunohistochemistry: Formalin-fixed and paraffin-embedded human pancreatic adenocarcinoma stained with ARG23870 anti-N Cadherin antibody.

  • ARG23870 anti-N Cadherin antibody WB image

    Western blot: Human endothelial cells untreated (left) or treated with Pervanadate (1 mM) for 30 min (right). The blots were stained with ARG23870 anti-N Cadherin antibody. (Observed MW: ~130 kDa)

  • ARG23870 anti-N Cadherin antibody IP image

    Immunoprecipitation: Mouse brain lysate immunoprecipitated with no antibody (lane 1), ARG23870 anti-N Cadherin antibody (lane 2) and whole mouse brain lysate (lane 3). The blot was stained with anti-N Cadherin (Cytoplasmic) Mouse monoclonal antibody (lanes 1-3).

客户反馈

nuts_pic      Excellent

anti-N Cadherin antibody

Application:WB

Sample:Mouse Brain

Sample Loading Amount:20 µg

Primary Antibody Dilution Factor:1:1000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC