ARG22587

anti-N Cadherin antibody [13A9]

anti-N Cadherin antibody [13A9] for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,IHC-Frozen sections,Immunoprecipitation,Western blot and Human,Mouse,Rat

EMT Study antibody; Mesenchymal Markers antibody
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anti-N Cadherin antibody [13A9]

Mouse Monoclonal antibody [13A9] recognizes Cadherin-N/N-Cadherin
This antibody recognizes neural cadherin, otherwise known as CD325, a calcium dependent cell-cell adhesion glycoprotein, and member of the cadherin superfamily, which links to the actin cytoskeleton via catenins, and plays a role in cell-matrix adhesion, cell growth and differentiation, and the establishment of left-right asymmetry.N-cadherin is expressed by neurons, endothelial cells, muscle cells, and stem cells, and is one of the primary cadherins recruited to the site of neuronal synapse formation. N-cadherin is directly involved in the differentiation of early hematopoietic progenitors, and is commonly expressed by cancer cells, playing a role in transendothelial migration and metastasis, through the up-regulation of the src kinase pathway, and subsequent failure of the intercellular connection between two adjacent endothelial cells. Mouse anti Human N-cadherin antibody, clone 13A9 studies have demonstrated that expression levels of E-Cadherin and N-Cadherin have a role to play in the invasive properties of breast cancer. Decreased levels of E-cadherin and loss of E-cadherin-mediated adhesion, can result in the transition of a benign epithelial tumor to an invasive tumor, and increase invasiveness, whilst the expression of N-cadherin correlates with motility, invasiveness and tumor metastasis, irrespective of the presence of E-cadherin (Nieman et al. 1999).Mouse anti Human N-cadherin antibody, clone 13A9 has been shown to be specific for N-cadherin, and does not recognize E-cadherin, M-cadherin or P-cadherin (Knudsen et al. 1995). Immunohistological studies have shown that clone 13A9 can be used as a reliable marker for the differential diagnosis of pleural mesotheliomas and lung adenocarcinomas, when used in conjunction with E-cadherin (Han et al. 1997).

ARG22587 Arigo biolaboratories
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anti-N Cadherin antibody [13A9] is rated 5.0 out of 5 by 2.

anti-N Cadherin antibody [13A9]

概述

产品描述

Mouse Monoclonal antibody [13A9] recognizes Cadherin-N/N-Cadherin
This antibody recognizes neural cadherin, otherwise known as CD325, a calcium dependent cell-cell adhesion glycoprotein, and member of the cadherin superfamily, which links to the actin cytoskeleton via catenins, and plays a role in cell-matrix adhesion, cell growth and differentiation, and the establishment of left-right asymmetry.N-cadherin is expressed by neurons, endothelial cells, muscle cells, and stem cells, and is one of the primary cadherins recruited to the site of neuronal synapse formation. N-cadherin is directly involved in the differentiation of early hematopoietic progenitors, and is commonly expressed by cancer cells, playing a role in transendothelial migration and metastasis, through the up-regulation of the src kinase pathway, and subsequent failure of the intercellular connection between two adjacent endothelial cells. Mouse anti Human N-cadherin antibody, clone 13A9 studies have demonstrated that expression levels of E-Cadherin and N-Cadherin have a role to play in the invasive properties of breast cancer. Decreased levels of E-cadherin and loss of E-cadherin-mediated adhesion, can result in the transition of a benign epithelial tumor to an invasive tumor, and increase invasiveness, whilst the expression of N-cadherin correlates with motility, invasiveness and tumor metastasis, irrespective of the presence of E-cadherin (Nieman et al. 1999).Mouse anti Human N-cadherin antibody, clone 13A9 has been shown to be specific for N-cadherin, and does not recognize E-cadherin, M-cadherin or P-cadherin (Knudsen et al. 1995). Immunohistological studies have shown that clone 13A9 can be used as a reliable marker for the differential diagnosis of pleural mesotheliomas and lung adenocarcinomas, when used in conjunction with E-cadherin (Han et al. 1997).

反应物种 Hu, Ms, Rat
应用 ICC/IF, IHC-Fr, IHC-P, IP, WB
宿主 Mouse
克隆 Monoclonal
克隆号 13A9
同位型 IgG1
靶点名称 N Cadherin
抗原物种 Human
抗原 Recombinant MBP fusion protein containing the entire cytoplasmic domain of human N-cadherin.
偶联标记 Un-conjugated
全名 cadherin 2
別名 Neural cadherin; N-cadherin; CD antigen CD325; Cadherin-2

应用说明

应用建议
应用 推荐稀释比
ICC/IF1:50-1:200
IHC-Fr1:50-1:200
IHC-P1:50-1:200
IP2 µg
WB1:100 - 1:1000
应用说明 IHC-P: This product requires antigen retrieval using steam heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
Western Blotting: MCA5698 detects a band of approximately 135-140kDa in human HT-1080 and HeLa cell lysates.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
预测分子量 100 kDa

属性

形式 Liquid
纯化 Purification with Protein G.
缓冲液 PBS and 0.09% Sodium azide
抗菌剂 0.09% Sodium azide
浓度 1 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 1000 Human CDH2

GeneID: 12558 Mouse CDH2

GeneID: 83501 Rat CDH2

基因名称 Cdh2
基因全名 cadherin 2
背景介绍 N Cadherin is a classical cadherin and member of the cadherin superfamily. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein is proteolytically processed to generate a calcium-dependent cell adhesion molecule and glycoprotein. This protein plays a role in the establishment of left-right asymmetry, development of the nervous system and the formation of cartilage and bone. [provided by RefSeq, Nov 2015]
生物功能 N Cadherin is a calcium-dependent cell adhesion protein; preferentially mediates homotypic cell-cell adhesion by dimerization with a CDH2 chain from another cell. Cadherins may thus contribute to the sorting of heterogeneous cell types. Acts as a regulator of neural stem cells quiescence by mediating anchorage of neural stem cells to ependymocytes in the adult subependymal zone: upon cleavage by MMP24, CDH2-mediated anchorage is affected, leading to modulate neural stem cell quiescence. CDH2 may be involved in neuronal recognition mechanism. In hippocampal neurons, may regulate dendritic spine density. [UniProt]
研究领域 EMT Study antibody; Mesenchymal Markers antibody
翻译后修饰 Cleaved by MMP24. Ectodomain cleavage leads to the generation of a soluble 90 kDa amino-terminal soluble fragment and a 45 kDa membrane-bound carboxy-terminal fragment 1 (CTF1), which is further cleaved by gamma-secretase into a 35 kDa. Cleavage in neural stem cells by MMP24 affects CDH2-mediated anchorage of neural stem cells to ependymocytes in the adult subependymal zone, leading to modulate neural stem cell quiescence (By similarity).
May be phosphorylated by OBSCN.

检测图片 (3) Click the Picture to Zoom In

  • ARG22587 anti-N Cadherin antibody [13A9] WB image

    Western blot: 20 µg of Mouse brain lysate stained with ARG22587 anti-N Cadherin antibody [13A9] at 1:1000 dilution.

  • ARG22587 anti-Cadherin-N/N-Cadherin antibody [13A9] IHC-P image

    Immunohistochemistry: Formalin-fixed and paraffin-embedded Human breast cancer biopsy stained with ARG22587 anti-Cadherin-N/N-Cadherin antibody [13A9] followed by HRP polymer detection and DAB substrate development. Antigen Retrieval: Boil tissue section in citrate buffer (pH 6.2). (low power).

  • ARG22587 anti-Cadherin-N/N-Cadherin antibody [13A9] IHC-P image

    Immunohistochemistry: Formalin-fixed and paraffin-embedded Human breast cancer biopsy stained with ARG22587 anti-Cadherin-N/N-Cadherin antibody [13A9] followed by HRP polymer detection and DAB substrate development. Antigen Retrieval: Boil tissue section in citrate buffer (pH 6.2). (high power).

客户反馈

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anti-N Cadherin antibody [13A9]

Application:WB

Sample:DLD-1, HCT 116 and HT 29

Sample Loading Amount:20 μg

Primary Antibody Dilution Factor:1:1000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

Conjugation of Secondary Antibody:HRP

nuts_pic      Excellent

anti-N Cadherin antibody [13A9]

Application:WB

Sample:Mouse brain

Sample Loading Amount:20 µg

Primary Antibody Dilution Factor:1:1000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC