ARG51616

anti-Myosin Light Chain 9 phospho (Ser19) antibody

anti-Myosin Light Chain 9 phospho (Ser19) antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat

Signaling Transduction antibody

概述

产品描述 Rabbit Polyclonal antibody recognizes Myosin Light Chain 9 phospho (Ser19)
反应物种 Hu, Ms, Rat
应用 IHC-P, WB
宿主 Rabbit
克隆 Polyclonal
同位型 IgG
靶点名称 Myosin Light Chain 9
抗原物种 Human
抗原 Peptide sequence around phosphorylation site of serine 19 (A-T-S(p)-N-V) derived from Human Myosin Light Chain 9. (NP_006088.2)
偶联标记 Un-conjugated
別名 LC20; Myosin RLC; 20 kDa myosin light chain; Myosin regulatory light chain 2, smooth muscle isoform; MYRL2; MLC2; Myosin regulatory light chain MRLC1; Myosin regulatory light chain 9; Myosin regulatory light polypeptide 9; MRLC1; MLC-2C

应用说明

应用建议
应用 推荐稀释比
IHC-P1:50 - 1:100
WB1:500 - 1:1000
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式 Liquid
纯化 Antibodies were produced by immunizing rabbits with KLH-conjugated synthetic phosphopeptide. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. In addition, non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
缓冲液 PBS (without Mg2+ and Ca2+, pH 7.4), 150mM NaCl, 0.02% Sodium azide and 50% Glycerol.
抗菌剂 0.02% Sodium azide
稳定剂 50% Glycerol
浓度 1 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 10398 Human MYL9

GeneID: 296313 Rat MYL9

GeneID: 98932 Mouse MYL9

基因名称 MYL9
全名 myosin, light chain 9, regulatory
背景介绍 Myosin regulatory subunit that plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. Implicated in cytokinesis, receptor capping, and cell locomotion
研究领域 Signaling Transduction antibody
预测分子量 20 kDa
翻译后修饰 Phosphorylation increases the actin-activated myosin ATPase activity and thereby regulates the contractile activity. It is required to generate the driving force in the migration of the cells but not necessary for localization of myosin-2 at the leading edge.

检测图片 (2) Click the Picture to Zoom In

  • ARG51616 anti-Myosin Light Chain 9 phospho (Ser19) antibody WB image

    Western blot: Extracts from 293 cells untreated or treated with starvation stained with ARG51616 anti-Myosin Light Chain 9 phospho (Ser19) antibody.

  • ARG51616 anti-Myosin Light Chain 9 phospho (Ser19) antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human breast carcinoma tissue stained with ARG51616 anti-Myosin Light Chain 9 phospho (Ser19) antibody (left) or the same antibody preincubated with blocking peptide (right).