ARG66503
anti-MyoD antibody
anti-MyoD antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
概述
| 产品描述 | Mouse Monoclonal antibody recognizes MyoD |
|---|---|
| 反应物种 | Hu |
| 应用 | IHC-P, WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 同位型 | IgG |
| 靶点名称 | MyoD |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide derived from Human MyoD. |
| 偶联标记 | Un-conjugated |
| 別名 | PUM; MYF3; Myoblast determination protein 1; bHLHc1; Myogenic factor 3; MYOD; Myf-3; Class C basic helix-loop-helix protein 1 |
应用说明
| 应用建议 |
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| 应用说明 | IHC-P: Antigen Retrieval: Citrate buffer (pH 6.0) was used. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| 实际分子量 | ~ 45 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | PBS, 0.02% Sodium azide, 50% Glycerol and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol and 0.5% BSA |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | MYOD1 |
| 全名 | myogenic differentiation 1 |
| 背景介绍 | This gene encodes a nuclear protein that belongs to the basic helix-loop-helix family of transcription factors and the myogenic factors subfamily. It regulates muscle cell differentiation by inducing cell cycle arrest, a prerequisite for myogenic initiation. The protein is also involved in muscle regeneration. It activates its own transcription which may stabilize commitment to myogenesis. [provided by RefSeq, Jul 2008] |
| 生物功能 | Acts as a transcriptional activator that promotes transcription of muscle-specific target genes and plays a role in muscle differentiation. Together with MYF5 and MYOG, co-occupies muscle-specific gene promoter core region during myogenesis. Induces fibroblasts to differentiate into myoblasts. Interacts with and is inhibited by the twist protein. This interaction probably involves the basic domains of both proteins (By similarity). [UniProt] |
| 细胞定位 | Nucleus. [UniProt] |
| 预测分子量 | 35 kDa |
| 翻译后修饰 | Phosphorylated by CDK9. This phosphorylation promotes its function in muscle differentiation. Acetylated by a complex containing EP300 and PCAF. The acetylation is essential to activate target genes. Conversely, its deacetylation by SIRT1 inhibits its function (By similarity). Ubiquitinated on the N-terminus; which is required for proteasomal degradation. Methylation at Lys-104 by EHMT2/G9a inhibits myogenic activity. [UniProt] |
检测图片 (2) Click the Picture to Zoom In
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ARG66503 anti-MyoD antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human rhabdomyosarcoma stained with ARG66503 anti-MyoD antibody at 1:200 (4°C, overnight). Antigen Retrieval: Citrate buffer (pH 6.0) was used.
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ARG66503 anti-MyoD antibody WB image
Western blot: 30 µg of HeLa and U2OS whole cell lysates stained with ARG66503 anti-MyoD antibody at 1:1000 dilution.
