anti-Myelin Basic Protein antibody [SQab19166]

anti-Myelin Basic Protein antibody [SQab19166] for IHC-Formalin-fixed paraffin-embedded sections and Human


产品描述 Recombinant Rabbit Monoclonal antibody [SQab19166] recognizes Myelin Basic Protein
反应物种 Hu
应用 IHC-P
宿主 Rabbit
克隆 Monoclonal
克隆号 SQab19166
同位型 IgG
靶点名称 Myelin Basic Protein
抗原物种 Human
抗原 Synthetic peptide within aa. 150-250 of Human Myelin Basic Protein.
偶联标记 Un-conjugated
別名 Myelin A1 protein; MBP; Myelin membrane encephalitogenic protein; Myelin basic protein


应用 推荐稀释比
IHC-P1:100 - 1:200
应用说明 IHC-P: Antigen Retrieval: Heat mediation was performed in Tris/EDTA buffer (pH 9.0), primary antibody incubate at RT (18°C-25°C) for 30 minutes.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.


形式 Liquid
纯化 Purification with Protein A.
缓冲液 PBS, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA.
抗菌剂 0.01% Sodium azide
稳定剂 40% Glycerol and 0.05% BSA
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.



GeneID: 4155 Human MBP

Swiss-port # P02686 Human Myelin basic protein

基因名称 MBP
全名 myelin basic protein
背景介绍 The protein encoded by the classic MBP gene is a major constituent of the myelin sheath of oligodendrocytes and Schwann cells in the nervous system. However, MBP-related transcripts are also present in the bone marrow and the immune system. These mRNAs arise from the long MBP gene (otherwise called "Golli-MBP") that contains 3 additional exons located upstream of the classic MBP exons. Alternative splicing from the Golli and the MBP transcription start sites gives rise to 2 sets of MBP-related transcripts and gene products. The Golli mRNAs contain 3 exons unique to Golli-MBP, spliced in-frame to 1 or more MBP exons. They encode hybrid proteins that have N-terminal Golli aa sequence linked to MBP aa sequence. The second family of transcripts contain only MBP exons and produce the well characterized myelin basic proteins. This complex gene structure is conserved among species suggesting that the MBP transcription unit is an integral part of the Golli transcription unit and that this arrangement is important for the function and/or regulation of these genes. [provided by RefSeq, Jul 2008]
生物功能 The classic group of MBP isoforms (isoform 4-isoform 14) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The smaller isoforms might have an important role in remyelination of denuded axons in multiple sclerosis. The non-classic group of MBP isoforms (isoform 1-isoform 3/Golli-MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T-cells and neural cells. Differential splicing events combined with optional post-translational modifications give a wide spectrum of isomers, with each of them potentially having a specialized function. Induces T-cell proliferation. [UniProt]
细胞定位 Myelin membrane; Peripheral membrane protein; Cytoplasmic side. Note=Cytoplasmic side of myelin. Isoform 3: Nucleus. Note=Targeted to nucleus in oligodendrocytes. [UniProt]
预测分子量 33 kDa
翻译后修饰 Several charge isomers of MBP; C1 (the most cationic, least modified, and most abundant form), C2, C3, C4, C5, C6, C7, C8-A and C8-B (the least cationic form); are produced as a result of optional PTM, such as phosphorylation, deamidation of glutamine or asparagine, arginine citrullination and methylation. C8-A and C8-B contain each two mass isoforms termed C8-A(H), C8-A(L), C8-B(H) and C8-B(L), (H) standing for higher and (L) for lower molecular weight. C3, C4 and C5 are phosphorylated. The ratio of methylated arginine residues decreases during aging, making the protein more cationic.

The N-terminal alanine is acetylated (isoform 3, isoform 4, isoform 5 and isoform 6).

Arg-241 was found to be 6% monomethylated and 60% symmetrically dimethylated.

Phosphorylated by TAOK2, VRK2, MAPK11, MAPK12, MAPK14 and MINK1. [UniProt]

检测图片 (1) Click the Picture to Zoom In

  • ARG66647 anti-Myelin Basic Protein antibody [SQab19166] IHC-P image

    Immunohistochemistry: Formalin/PFA-fixed and paraffin-embedded Human glioma tissue stained with ARG66647 anti-Myelin Basic Protein antibody [SQab19166]. Antigen Retrieval: Heat mediation was performed in Tris/EDTA buffer (pH 9.0).