ARG11119
anti-Myelin Basic Protein antibody
anti-Myelin Basic Protein antibody for IHC-Frozen sections,Western blot and Cow,Human,Mouse,Pig,Rat
概述
| 产品描述 | Goat Polyclonal antibody recognizes Myelin Basic Protein |
|---|---|
| 反应物种 | Hu, Ms, Rat, Cow, Pig |
| 应用 | IHC-Fr, WB |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | Myelin Basic Protein |
| 抗原物种 | Bovine |
| 抗原 | Purified Myelin Basic Protein isolated from bovine brain. |
| 偶联标记 | Un-conjugated |
| 別名 | MBP; Myelin Basic Protein; Myelin Membrane Encephalitogenic Protein; Myelin A1 Protein; Golli-MBP |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified. |
| 缓冲液 | PBS, 5 mM Sodium azide and 50% Glycerol. |
| 抗菌剂 | 5 mM Sodium azide |
| 稳定剂 | 50% Glycerol |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | MBP |
| 全名 | Myelin Basic Protein |
| 背景介绍 | The protein encoded by the classic MBP gene is a major constituent of the myelin sheath of oligodendrocytes and Schwann cells in the nervous system. However, MBP-related transcripts are also present in the bone marrow and the immune system. These mRNAs arise from the long MBP gene (otherwise called "Golli-MBP") that contains 3 additional exons located upstream of the classic MBP exons. Alternative splicing from the Golli and the MBP transcription start sites gives rise to 2 sets of MBP-related transcripts and gene products. The Golli mRNAs contain 3 exons unique to Golli-MBP, spliced in-frame to 1 or more MBP exons. They encode hybrid proteins that have N-terminal Golli aa sequence linked to MBP aa sequence. The second family of transcripts contain only MBP exons and produce the well characterized myelin basic proteins. This complex gene structure is conserved among species suggesting that the MBP transcription unit is an integral part of the Golli transcription unit and that this arrangement is important for the function and/or regulation of these genes. [provided by RefSeq, Jul 2008] |
| 生物功能 | The classic group of MBP isoforms (isoform 4-isoform 14) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The smaller isoforms might have an important role in remyelination of denuded axons in multiple sclerosis. The non-classic group of MBP isoforms (isoform 1-isoform 3/Golli-MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T-cells and neural cells. Differential splicing events combined with optional post-translational modifications give a wide spectrum of isomers, with each of them potentially having a specialized function. Induces T-cell proliferation. [UniProt] |
| 细胞定位 | Cell membrane; Membrane; Nucleus. [UniProt] |
| 预测分子量 | 33 kDa |
| 翻译后修饰 | Acetylation; Citrullination; Methylation; Phosphoprotein. [UniProt] |
检测图片 (3) Click the Picture to Zoom In
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ARG11119 anti-Myelin Basic Protein antibody IHC-Fr image
Immunohistochemistry: Frozen section of Mouse hippocampus tissue stained with ARG11119 anti-Myelin Basic Protein antibody (red) at 1:5000 dilution, and co-stained with anti-GFAP antibody (green) at 1:5000 dilution. Hoechst (blue) for nuclear staining.
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ARG11119 anti-Myelin Basic Protein antibody WB image
Western blot: Rat cerebellum, Mouse cerebellum, Cow midbrain and Pig midbrain lysates stained with ARG11119 anti-Myelin Basic Protein antibody at 1:5000 dilution.
Multiple bands between 15-25 kDa mark correspond to the various alternate transcripts of the single MBP gene.
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ARG11119 anti-Myelin Basic Protein antibody IHC-Fr image
Immunohistochemistry: Frozen section of Rat cerebellum tissue stained with ARG11119 anti-Myelin Basic Protein antibody (red) at 1:5000 dilution, and co-stained with anti-GFAP antibody (green) at 1:5000 dilution.
