ARG53920
anti-Myc tag antibody [9E10] (FITC)
anti-Myc tag antibody [9E10] (FITC) for Flow cytometry and Other
Cancer antibody; Controls and Markers antibody; Developmental Biology antibody; Gene Regulation antibody; Signaling Transduction antibody
概述
| 产品描述 | FITC-conjugated Mouse Monoclonal antibody [9E10] recognizes Myc tag |
|---|---|
| 反应物种 | Other |
| 应用 | FACS |
| 特异性 | The clone 9E10 may be used to detect the c-Myc tag. The c-myc gene (8q24 on human chromosome) is the cellular homologue of the v-myc gene originally isolated from an avian myelocytomatosis virus. The c-Myc protein is a transcription factor (nuclear localization). c-Myc is commonly activated in a variety of tumor cells and plays an important role in cellular proliferation, differentiation, apoptosis and cell cycle progression. The phosphorylation of c-Myc has been investigated and previous studies have suggested a functional association between phosphorylation at Thr58/Ser62 by glycogen synthase kinase 3, cyclin-dependent kinase, ERK2 and C-Jun N-terminal Kinase (JNK) in cell proliferation and cell cycle regulation. In normal cells the expression of c-Myc is tightly regulated but in human cancers c-Myc is frequently deregulated. c-Myc is also essential for tumor cell development in vasculogenesis and angiogenesis that distribute blood throughout the cells. |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | 9E10 |
| 同位型 | IgG1 |
| 靶点名称 | Myc tag |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide sequence (AEEQKLISEEDLL) corresponding to the C-terminal region of human Myc |
| 偶联标记 | FITC |
| 別名 | c-Myc; MRTL; MYCC; Class E basic helix-loop-helix protein 39; Proto-oncogene c-Myc; bHLHe39; Myc proto-oncogene protein; Transcription factor p64 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化说明 | The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC. |
| 缓冲液 | PBS (pH 7.4) and 15 mM Sodium azide |
| 抗菌剂 | 15 mM Sodium azide |
| 浓度 | 1 mg/ml |
| 存放说明 | Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 基因名称 | MYC |
|---|---|
| 全名 | v-myc avian myelocytomatosis viral oncogene homolog |
| 背景介绍 | The protein encoded by this gene is a multifunctional, nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. It functions as a transcription factor that regulates transcription of specific target genes. Mutations, overexpression, rearrangement and translocation of this gene have been associated with a variety of hematopoietic tumors, leukemias and lymphomas, including Burkitt lymphoma. There is evidence to show that alternative translation initiations from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site result in the production of two isoforms with distinct N-termini. The synthesis of non-AUG initiated protein is suppressed in Burkitt's lymphomas, suggesting its importance in the normal function of this gene. [provided by RefSeq, Jul 2008] |
| 生物功能 | Transcription factor that binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3'. Activates the transcription of growth-related genes. [UniProt] |
| 研究领域 | Cancer antibody; Controls and Markers antibody; Developmental Biology antibody; Gene Regulation antibody; Signaling Transduction antibody |
| 翻译后修饰 | Phosphorylated by PRKDC. Phosphorylation at Ser-329 by PIM2 leads to the stabilization of MYC (By similarity). Phosphorylation at Ser-62 by CDK2 prevents Ras-induced senescence. Phosphorylated at Ser-62 by DYRK2; this primes the protein for subsequent phosphorylation by GSK3B at Thr-58. Phosphorylation at Thr-58 and Ser-62 by GSK3 is required for ubiquitination and degradation by the proteasome. Ubiquitinated by the SCF(FBXW7) complex when phosphorylated at Thr-58 and Ser-62, leading to its degradation by the proteasome. In the nucleoplasm, ubiquitination is counteracted by USP28, which interacts with isoform 1 of FBXW7 (FBW7alpha), leading to its deubiquitination and preventing degradation. In the nucleolus, however, ubiquitination is not counteracted by USP28, due to the lack of interaction between isoform 4 of FBXW7 (FBW7gamma) and USP28, explaining the selective MYC degradation in the nucleolus. Also polyubiquitinated by the DCX(TRUSS) complex. Ubiquitinated by TRIM6 in a phosphorylation-independent manner (By similarity). |
检测图片 (2) Click the Picture to Zoom In
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ARG53920 anti-Myc tag antibody [9E10] (FITC) FACS image
Flow Cytometry: LST1-Myc transfected HEK293 cells (red) and nontransfected HEK293 cells (blue) stained with ARG53920 anti-Myc tag antibody [9E10] (FITC).
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ARG53920 anti-Myc tag antibody [9E10] (FITC) FACS image
Flow Cytometry: LST1-Myc transfected HEK293 cells (red) and nontransfected HEK293 cells (black-dashed) stained with ARG53920 anti-Myc tag antibody [9E10] (FITC).
克隆号文献
The 9-1-1 checkpoint clamp stimulates DNA resection by Dna2-Sgs1 and Exo1.
IP /
SIRT1 mediates FOXA2 breakdown by deacetylation in a nutrient-dependent manner.
WB / Human
The diabetes gene Hhex maintains δ-cell differentiation and islet function.
ChIP /
c-Myc-mediated repression of miR-15-16 in hypoxia is induced by increased HIF-2α and promotes tumor angiogenesis and metastasis by upregulating FGF2.
Isolation and characterisation of a recombinant antibody fragment that binds NCAM1-expressing intervertebral disc cells.
ELISA /
The ciliary protein cystin forms a regulatory complex with necdin to modulate Myc expression.
WB, IP /
Resolution by unassisted Top3 points to template switch recombination intermediates during DNA replication.
WB /
SUMO localizes to the central element of synaptonemal complex and is required for the full synapsis of meiotic chromosomes in budding yeast.
IHC-Fr / Saccharomyces cerevisiae
Binding of TFIIIC to sine elements controls the relocation of activity-dependent neuronal genes to transcription factories.
ICC/IF / Mouse
Shank3-Rich2 interaction regulates AMPA receptor recycling and synaptic long-term potentiation.
Activation of a PGC-1-related coactivator (PRC)-dependent inflammatory stress program linked to apoptosis and premature senescence.
WB / Human
Regulation of fertility, survival, and cuticle collagen function by the Caenorhabditis elegans eaf-1 and ell-1 genes.
WB / Invertebrates
Differential expression of MYC in H. pylori-related intestinal and diffuse gastric tumors.
IHC / Human
SUMOylation promotes de novo targeting of HP1α to pericentric heterochromatin.
WB / Mouse
Antibody library screens using detergent-solubilized mammalian cell lysates as antigen sources.
FACS / Human
Limited functional and metabolic improvements in hypertrophic and healthy rat heart overexpressing the skeletal muscle isoform of SERCA1 by adenoviral gene transfer in vivo.
WB / Rat
The Drosophila protein palmitoylome: characterizing palmitoyl-thioesterases and DHHC palmitoyl-transferases.
ICC/IF / Fruit fly (Drosophila melanogaster)
Molecular cloning of caveolin-3, a novel member of the caveolin gene family expressed predominantly in muscle.
WB / Rat
SNAP receptors implicated in vesicle targeting and fusion.
IP / Bovine
A conditional allele of the novel repeat-containing yeast nucleoporin RAT7/NUP159 causes both rapid cessation of mRNA export and reversible clustering of nuclear pore complexes.
ICC/IF / Yeast
