ARG51785
anti-Myc phospho (Ser62) antibody
anti-Myc phospho (Ser62) antibody for ICC/IF,Western blot and Human,Mouse,Rat
Cancer antibody; Controls and Markers antibody; Developmental Biology antibody; Gene Regulation antibody; Signaling Transduction antibody
1
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes Myc phospho (Ser62) |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | ICC/IF, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | Myc |
| 抗原物种 | Human |
| 抗原 | KLH-conjugated synthetic phosphopeptide around Ser62 (P-L-S(p)-P-S) of Human Myc. |
| 偶联标记 | Un-conjugated |
| 別名 | c-Myc; MRTL; MYCC; Class E basic helix-loop-helix protein 39; Proto-oncogene c-Myc; bHLHe39; Myc proto-oncogene protein; Transcription factor p64 |
应用说明
| 应用建议 |
|
||||||
|---|---|---|---|---|---|---|---|
| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with phospho-specific peptide and the non-phospho specific antibodies were removed by chromatography using non-phosphopeptide. |
| 缓冲液 | PBS (without Mg2+ and Ca2+, pH 7.4), 150mM NaCl, 0.02% Sodium azide and 50% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | MYC |
| 全名 | v-myc avian myelocytomatosis viral oncogene homolog |
| 背景介绍 | Participates in the regulation of gene transcription. Binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3'. Seems to activate the transcription of growth-related genes. |
| 生物功能 | Transcription factor that binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3'. Activates the transcription of growth-related genes. [UniProt] |
| 产品亮点 | Related Antibody Duos and Panels: ARG30012 Phospho Myc Antibody Duo (pS62, pS58) ARG30125 Phospho c-Myc Antibody Duo (Total, pS62) ARG30126 Phospho c-Myc Antibody Panel (Total, pT58, pS62) Related products: Myc antibodies; Myc ELISA Kits; Myc Duos / Panels; Anti-Rabbit IgG secondary antibodies; |
| 研究领域 | Cancer antibody; Controls and Markers antibody; Developmental Biology antibody; Gene Regulation antibody; Signaling Transduction antibody |
| 预测分子量 | 49 kDa |
| 翻译后修饰 | Phosphorylated by PRKDC. Phosphorylation at Ser-329 by PIM2 leads to the stabilization of MYC (By similarity). Phosphorylation at Ser-62 by CDK2 prevents Ras-induced senescence. Phosphorylated at Ser-62 by DYRK2; this primes the protein for subsequent phosphorylation by GSK3B at Thr-58. Phosphorylation at Thr-58 and Ser-62 by GSK3 is required for ubiquitination and degradation by the proteasome. Ubiquitinated by the SCF(FBXW7) complex when phosphorylated at Thr-58 and Ser-62, leading to its degradation by the proteasome. In the nucleoplasm, ubiquitination is counteracted by USP28, which interacts with isoform 1 of FBXW7 (FBW7alpha), leading to its deubiquitination and preventing degradation. In the nucleolus, however, ubiquitination is not counteracted by USP28, due to the lack of interaction between isoform 4 of FBXW7 (FBW7gamma) and USP28, explaining the selective MYC degradation in the nucleolus. Also polyubiquitinated by the DCX(TRUSS) complex. Ubiquitinated by TRIM6 in a phosphorylation-independent manner (By similarity). |
检测图片 (2) Click the Picture to Zoom In
-
ARG51785 anti-Myc phospho (Ser62) antibody WB image
Western blot: 30 µg of HeLa cell lysates untreated or treated with calyculin A (50nM, 30mins). The blots were stained with ARG51785 anti-Myc phospho (Ser62) antibody at 1:500 dilution.
-
ARG51785 anti-Myc phospho (Ser62) antibody ICC/IF image
Immunofluorescence: methanol-fixed HeLa cells stained with ARG51785 anti-Myc phospho (Ser62) antibody.
客户反馈
Excellent
anti-Myc phospho (Ser62) antibody
Application：WB
Sample：HeLa untreated or treated with calyculin A (50nM, 30mins)
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：1:500
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
