ARG64655
anti-Monoglyceride Lipase antibody
anti-Monoglyceride Lipase antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
Cancer antibody; Cell Biology and Cellular Response antibody; Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody
概述
| 产品描述 | Goat Polyclonal antibody recognizes Monoglyceride Lipase |
|---|---|
| 反应物种 | Hu, Ms |
| 预测物种 | Cow, Dog |
| 应用 | IHC-P, WB |
| 特异性 | This antibody is expected to recognize both reported isoforms (NP_009214.1; NP_001003794.1). |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | Monoglyceride Lipase |
| 抗原物种 | Human |
| 抗原 | C-QDLPHLVNADGQY |
| 偶联标记 | Un-conjugated |
| 別名 | Lysophospholipase-like; Monoacylglycerol lipase; EC 3.1.1.23; HUK5; Lysophospholipase homolog; MAGL; HU-K5; Monoglyceride lipase; MGL |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|---|---|
| 应用说明 | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from goat serum by antigen affinity chromatography. |
| 缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 背景介绍 | This gene encodes a serine hydrolase of the AB hydrolase superfamily that catalyzes the conversion of monoacylglycerides to free fatty acids and glycerol. The encoded protein plays a critical role in several physiological processes including pain and nociperception through hydrolysis of the endocannabinoid 2-arachidonoylglycerol. Expression of this gene may play a role in cancer tumorigenesis and metastasis. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Feb 2012] |
| 研究领域 | Cancer antibody; Cell Biology and Cellular Response antibody; Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody |
| 预测分子量 | 33 kDa |
检测图片 (3) Click the Picture to Zoom In
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ARG64655 anti-Monoglyceride Lipase antibody WB image
Western Blot: 1). Mock transfection; 2) MGLL (RC218358) expressing plasmid transfected HEK293 cell lysate standed with ARG64655 anti-Monoglyceride Lipase antibody
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ARG64655 anti-Monoglyceride Lipase antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human liver tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0). The tissue section was stained with ARG64655 anti-Monoglyceride Lipase antibody at 5 µg/ml dilution followed by HRP-staining.
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ARG64655 anti-Monoglyceride Lipase antibody WB image
Western blot: 35 µg of Human frontal cortex (A) and Mouse adipose (B) lysates (in RIPA buffer) stained with ARG64655 anti-Monoglyceride Lipase antibody at 0.5 µg/ml dilution and incubated at RT for 1 hour.
