ARG58005
anti-Mitofusin 1 antibody
anti-Mitofusin 1 antibody for ICC/IF,Western blot and Human
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes Mitofusin 1 |
|---|---|
| 反应物种 | Hu |
| 应用 | ICC/IF, WB |
| 特异性 | This antibody is predicted to not cross-react with Mitofusin 2. |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | Mitofusin 1 |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide corresponding to 17 aa (N-terminus) of Human Mitofusin 1. |
| 偶联标记 | Un-conjugated |
| 別名 | EC 3.6.5.-; Fzo homolog; Mitofusin-1; hfzo1; hfzo2; Transmembrane GTPase MFN1 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| 阳性对照 | WB: A431 cell lysate. | ||||||
| 实际分子量 | ~ 82 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | PBS and 0.02% Sodium azide. |
| 抗菌剂 | 0.02% Sodium azide |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | MFN1 |
| 全名 | mitofusin 1 |
| 背景介绍 | The protein encoded by this gene is a mediator of mitochondrial fusion. This protein and mitofusin 2 are homologs of the Drosophila protein fuzzy onion (Fzo). They are mitochondrial membrane proteins that interact with each other to facilitate mitochondrial targeting. [provided by RefSeq, Jul 2008] |
| 生物功能 | Essential transmembrane GTPase, which mediates mitochondrial fusion. Fusion of mitochondria occurs in many cell types and constitutes an important step in mitochondria morphology, which is balanced between fusion and fission. MFN1 acts independently of the cytoskeleton. Overexpression induces the formation of mitochondrial networks. [UniProt] |
| 预测分子量 | 84 kDa |
| 翻译后修饰 | Ubiquitinated by non-degradative ubiquitin by PRKN (PubMed:23933751). Deubiquitination by USP30 inhibits mitochondrial fusion (By similarity). Ubiquitinated by MARCH5 (PubMed:20103533). When mitochondria are depolarized and dysfunctional, it is ubiquitinated by a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex that contains FBXO7 and PRKN (PubMed:23933751). [UniProt] |
检测图片 (2) Click the Picture to Zoom In
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ARG58005 anti-Mitofusin 1 antibody ICC/IF image
Immunocytochemistry: A431 cells stained with ARG58005 anti-Mitofusin 1 antibody at 5 µg/ml dilution.
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ARG58005 anti-Mitofusin 1 antibody WB image
Western blot: A431 cell lysate stained with ARG58005 anti-Mitofusin 1 antibody at 1 µg/ml dilution.
