ARG52332
anti-MeCP2 phospho (Ser421) antibody
anti-MeCP2 phospho (Ser421) antibody for Western blot and Mouse
Gene Regulation antibody; Neuroscience antibody
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes MeCP2 phospho (Ser421) |
|---|---|
| 反应物种 | Ms |
| 预测物种 | Hu, NHuPrm |
| 应用 | WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | MeCP2 |
| 抗原物种 | Mouse |
| 抗原 | Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser421 conjugated to KLH |
| 偶联标记 | Un-conjugated |
| 別名 | MRXSL; RS; MeCp2; Methyl-CpG-binding protein 2; MeCp-2 protein; RTT; AUTSX3; RTS; MRX79; PPMX; MRXS13; MRX16 |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|
| 应用说明 | Specific for the ~55 kDa truncated MeCP2 protein phosphorylated at Ser421. Immunolabeling of the MeCP2 band is blocked by preadsorption with the phospho-peptide used as antigen but not by the corresponding dephospho-peptide. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity Purified |
| 缓冲液 | 10 mM HEPES (pH 7.5), 150 mM NaCl, 0.1 mg/ml BSA and 50% Glycerol |
| 稳定剂 | 0.1 mg/ml BSA, 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | MECP2 |
| 全名 | methyl CpG binding protein 2 |
| 背景介绍 | MeCP2 (Methyl-CpG Binding Protein 2) is a chromosomal protein that binds to methylated DNA. It can bind specifically to a single methyl-CpG pair and is not influenced by sequences flanking the methyl-CpGs. MeCP2 has been shown to mediate transcriptional repression through interaction with histone deacetylase and the corepressor SIN3A (Nan et al., 1998). Defects in MeCP2 are the cause of Rett syndrome (RTT) (Amir et al., 1999). RTT is an X-linked dominant disease; it is a progressive neurologic developmental disorder and one of the most common causes of mental retardation in females. Recent studies have reported a new phosphorylation site at Ser421. Phosphorylation and dephosphorylation of this site may be involved in regulation of behavioral responses to chronic antidepressant treatment (Hutchinson et al., 2012). |
| 研究领域 | Gene Regulation antibody; Neuroscience antibody |
| 预测分子量 | 52 kDa |
| 翻译后修饰 | Phosphorylated on Ser-423 in brain upon synaptic activity, which attenuates its repressor activity and seems to regulate dendritic growth and spine maturation. |
检测图片 (1) Click the Picture to Zoom In
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ARG52332 anti-MeCP2 phospho (Ser421) antibody WB image
Western blot: Mouse whole brain showing specific immunolabeling of the ~ 55 kDa truncated MeCP2 protein phosphorylated at Ser421 by using ARG52332 anti-MeCP2 phospho (Ser421) antibody.
Immunolabeling is greatly reduced by preadsorption with the phospho-peptide used as antigen (peptide).
