ARG10762
anti-MeCP2 phospho (Ser421) antibody [2E2]
anti-MeCP2 phospho (Ser421) antibody [2E2] for Dot blot,ICC/IF,Immunohistochemistry (PFA perfusion fixed frozen sections) and Mouse
概述
| 产品描述 | Mouse Monoclonal antibody [2E2] recognizes MeCP2 phospho (Ser421) |
|---|---|
| 反应物种 | Ms |
| 应用 | Dot, ICC/IF, IHC-FoFr |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | 2E2 |
| 同位型 | IgG1 |
| 靶点名称 | MeCP2 |
| 抗原物种 | Human |
| 抗原 | KLH-conjugated synthetic phosphopeptide around Ser421 of Human MeCP2. |
| 偶联标记 | Un-conjugated |
| 別名 | MRXSL; RS; MeCp2; Methyl-CpG-binding protein 2; MeCp-2 protein; RTT; AUTSX3; RTS; MRX79; PPMX; MRXS13; MRX16 |
应用说明
| 应用建议 |
|
||||||||
|---|---|---|---|---|---|---|---|---|---|
| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification. |
| 缓冲液 | PBS and 50% Glycerol. |
| 稳定剂 | 50% Glycerol |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | MECP2 |
| 全名 | methyl CpG binding protein 2 |
| 背景介绍 | DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. In contrast to other MBD family members, MECP2 is X-linked and subject to X inactivation. MECP2 is dispensible in stem cells, but is essential for embryonic development. MECP2 gene mutations are the cause of most cases of Rett syndrome, a progressive neurologic developmental disorder and one of the most common causes of mental retardation in females. [provided by RefSeq, Jul 2009] |
| 生物功能 | Chromosomal protein that binds to methylated DNA. It can bind specifically to a single methyl-CpG pair. It is not influenced by sequences flanking the methyl-CpGs. Mediates transcriptional repression through interaction with histone deacetylase and the corepressor SIN3A. Binds both 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC)-containing DNA, with a preference for 5-methylcytosine (5mC). [UniProt] |
| 预测分子量 | 52 kDa |
| 翻译后修饰 | Phosphorylated on Ser-423 in brain upon synaptic activity, which attenuates its repressor activity and seems to regulate dendritic growth and spine maturation. |
检测图片 (2) Click the Picture to Zoom In
-
ARG10762 anti-MeCP2 phospho (Ser421) antibody [2E2] ICC/IF image
Immunocytochemistry: Mouse brain section, which was cut at 45 µM on a vibratome after perfusion with 4% parformaldehyde and overnight fixation, was stained with ARG10762 anti-MeCP2 phospho (Ser421) antibody [2E2] at 1:1000 dilution. Phospho-MeCP2 at S421 was mainly associated with methylated DNA in nucleus of activated neuron cells (red). Blue shows DAPI staining of nuclear DNA. .
-
ARG10762 anti-MeCP2 phospho (Ser421) antibody [2E2] Dot image
Dot blot: 0.5 µg of Phospho-peptide or Non Phospho-peptide stained with ARG10762 anti-MeCP2 phospho (Ser421) antibody [2E2] on nitrocellulose membrane.
