ARG45659

anti-Mannose Phosphate Isomerase antibody [5G5]

anti-Mannose Phosphate Isomerase antibody [5G5] for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Rat

概述

产品描述 Mouse Monoclonal antibody [5G5] recognizes Mannose Phosphate Isomerase
反应物种 Hu, Rat
应用 FACS, ICC/IF, IHC-P, WB
宿主 Mouse
克隆 Monoclonal
克隆号 5G5
同位型 IgG2a
靶点名称 Mannose Phosphate Isomerase
抗原物种 Human
抗原 Recombinant protein containing to human Mannose Phosphate Isomerase.
偶联标记 Un-conjugated
別名 MPI; mannose phosphate isomerase; Phosphohexomutase; EC 5.3.1.8; PMI1; CDG1B; Phosphomannose isomerase; Mannose-6-phosphate isomerase; PMI

应用说明

应用建议
应用 推荐稀释比
FACS1 - 3 µg/10^6 cells
ICC/IF5 μg/ml
IHC-P2-5 μg/ml
WB0.25-0.5 μg/ml
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
实际分子量 45 kDa

属性

形式 Liquid
纯化 Affinity purified
缓冲液 0.2% Na2HPO4, 0.9% NaCl and 4% Trehalose.
稳定剂 4% Trehalose
浓度 0.5 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 4351 Human MPI

Swiss-port # P34949 Human Mannose-6-phosphate isomerase

基因名称 MPI
全名 mannose phosphate isomerase
背景介绍 Phosphomannose isomerase catalyzes the interconversion of fructose-6-phosphate and mannose-6-phosphate and plays a critical role in maintaining the supply of D-mannose derivatives, which are required for most glycosylation reactions. Mutations in the MPI gene were found in patients with carbohydrate-deficient glycoprotein syndrome, type Ib. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]
生物功能 Involved in the synthesis of the GDP-mannose and dolichol-phosphate-mannose required for a number of critical mannosyl transfer reactions. [UniProt]
细胞定位 Cytoplasm. [UniProt]
预测分子量 47 kDa
翻译后修饰 Acetylation; Phosphoprotein. [UniProt]

检测图片 (5) Click the Picture to Zoom In

  • ARG45659 anti-Mannose Phosphate Isomerase antibody [5G5] IHC-P image

    Immunohistochemistry: Human lung cancer stained with ARG45659 anti-Mannose Phosphate Isomerase antibody [5G5] at 2 μg/ml dilution.

  • ARG45659 anti-Mannose Phosphate Isomerase antibody [5G5] ICC/IF image

    Immunofluorescence: T-47D stained with ARG45659 anti-Mannose Phosphate Isomerase antibody [5G5] at 5 μg/ml dilution.

  • ARG45659 anti-Mannose Phosphate Isomerase antibody [5G5] WB image

    Western blot: Hela stained with ARG45659 anti-Mannose Phosphate Isomerase antibody [5G5] at 0.5 μg/ml dilution.

  • ARG45659 anti-Mannose Phosphate Isomerase antibody [5G5] FACS image

    Flow Cytometry: U87 stained with ARG45659 anti-Mannose Phosphate Isomerase antibody [5G5] at 1 µg/10^6 cells dilution.

  • ARG45659 anti-Mannose Phosphate Isomerase antibody [5G5] WB image

    Western blot: Rat brain stained with ARG45659 anti-Mannose Phosphate Isomerase antibody [5G5] at 0.5 μg/ml dilution.