ARG59747
anti-MafA antibody
anti-MafA antibody for Western blot and Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes MafA |
|---|---|
| 反应物种 | Rat |
| 应用 | WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | MafA |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide corresponding to aa. 136-167 of Human MafA. (EDAVEALIGSGHHGAHHGAHHPAAAAAYEAFR) |
| 偶联标记 | Un-conjugated |
| 別名 | RIPE3b1; Transcription factor MafA; Transcription factor RIPE3b1; V-maf musculoaponeurotic fibrosarcoma oncogene homolog A; hMafA; Pancreatic beta-cell-specific transcriptional activator |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | 0.9% NaCl, 0.2% Na2HPO4, 0.05% Sodium azide and 5% BSA. |
| 抗菌剂 | 0.05% Sodium azide |
| 稳定剂 | 5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 基因名称 | MAFA |
|---|---|
| 全名 | v-maf avian musculoaponeurotic fibrosarcoma oncogene homolog A |
| 背景介绍 | MAFA is a transcription factor that binds RIPE3b, a conserved enhancer element that regulates pancreatic beta cell-specific expression of the insulin gene (INS; MIM 176730) (Olbrot et al., 2002 [PubMed 12011435]).[supplied by OMIM, Mar 2008] |
| 生物功能 | Acts as a transcriptional factor. Specifically binds the insulin enhancer element RIPE3b and activates insulin gene expression. Cooperates synergistically with NEUROD1 and PDX1. Phosphorylation by GSK3 increases its transcriptional activity and is required for its oncogenic activity. Involved either as an oncogene or as a tumor suppressor, depending on the cell context. [UniProt] |
| 细胞定位 | Nucleus. Note=Detected in nuclei of pancreas islet beta cells. [UniProt] |
| 预测分子量 | 37 kDa |
| 翻译后修饰 | Ubiquitinated, leading to its degradation by the proteasome. Ser-14 and Ser-65 appear to be the major phosphorylation sites. Phosphorylated by MAPK13 on serine and threonine residues (Probable). Phosphorylation by GSK3 requires prior phosphorylation of Ser-65 by another kinase. Phosphorylation proceeds then from Ser-61 to Thr-57, Thr-53 and Ser-49. GSK3-mediated phosphorylation increases its transcriptional activity through the recruitment of the coactivator PCAF, is required for its transforming activity and leads to its degradation through a ubiquitin/proteasome-dependent pathway. [UniProt] |
检测图片 (1) Click the Picture to Zoom In
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ARG59747 anti-MafA antibody WB image
Western blot: 50 µg of Rat cardiac muscle lysate stained with ARG59747 anti-MafA antibody at 0.5 µg/ml dilution.
