ARG22751
anti-Macrophage antibody [BA4D5]
anti-Macrophage antibody [BA4D5] for Flow cytometry,IHC-Frozen sections,Immunoprecipitation,Western blot and Pig
概述
| 产品描述 | Mouse Monoclonal antibody [BA4D5] recognizes Macrophage This antibody recognizes Porcine cells of the monocyte/macrophage lineage. Expression of the antigen is increased with maturation, with higher expression on peritoneal and alveolar macrophages. Some expression has also been observed on peripheral blood lymphocytes. The antigen recognized by clone BA4D5 has a broad tissue distribution and this antibody stains macrophages in a range of tissues, including the thymus, spleen periarteriolar lymphoid sheath (PALS), spleen red pulp and the Peyeras patches. Expression has also been reported on some non-heamatopoietic cells including endothelial cells. It is believed that clone BA4D5 may be specific for Porcine CD68, although the protein recognized by this antibody has not yet been fully characterized. The protein is expressed on the cell surface, although it is most abundantly expressed in the cytoplasm. |
|---|---|
| 反应物种 | Pig |
| 应用 | FACS, IHC-Fr, IP, WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | BA4D5 |
| 同位型 | IgG2b |
| 靶点名称 | Macrophage |
| 抗原物种 | Pig |
| 抗原 | Porcine alveolar macrophages. |
| 偶联标记 | Un-conjugated |
应用说明
| 应用建议 |
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| 应用说明 | WB: The antibody recognizes a 105 kDa antigen in pig macrophage lysates under non-reducing conditions. FACS: Membrane permeabilization is required for this application. Use 10 µl of the suggested working dilution to label 10^6 cells in 100 µl. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein G. |
| 缓冲液 | PBS and 0.09% Sodium azide. |
| 抗菌剂 | 0.09% Sodium azide |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
检测图片 (1) Click the Picture to Zoom In
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ARG22751 anti-Macrophage antibody [BA4D5] FACS image
Flow Cytometry: Pig peripheral blood monocytes stained with ARG22751 anti-Macrophage antibody [BA4D5] followed by Goat anti-Mouse IgG (FITC).
