ARG42116
anti-MYLIP / IDOL antibody
anti-MYLIP / IDOL antibody for Western blot and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes MYLIP / IDOL |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | MYLIP / IDOL |
| 抗原物种 | Human |
| 抗原 | Recombinant fusion protein corresponding to aa. 266-445 of Human MYLIP / IDOL (NP_037394.2). |
| 偶联标记 | Un-conjugated |
| 別名 | Inducible degrader of the LDL-receptor; EC 6.3.2.-; Myosin regulatory light chain interacting protein; E3 ubiquitin-protein ligase MYLIP; IDOL; MIR; Idol |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|
| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||
| 阳性对照 | Jurkat | ||||
| 实际分子量 | ~ 50 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified. |
| 缓冲液 | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | MYLIP |
| 全名 | myosin regulatory light chain interacting protein |
| 背景介绍 | The ERM protein family members ezrin, radixin, and moesin are cytoskeletal effector proteins linking actin to membrane-bound proteins at the cell surface. Myosin regulatory light chain interacting protein (MYLIP) is a novel ERM-like protein that interacts with myosin regulatory light chain and inhibits neurite outgrowth. [provided by RefSeq, Jul 2008] |
| 生物功能 | E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of myosin regulatory light chain (MRLC), LDLR, VLDLR and LRP8. Activity depends on E2 enzymes of the UBE2D family. Proteasomal degradation of MRLC leads to inhibit neurite outgrowth in presence of NGF by counteracting the stabilization of MRLC by saposin-like protein (CNPY2/MSAP) and reducing CNPY2-stimulated neurite outgrowth. Acts as a sterol-dependent inhibitor of cellular cholesterol uptake by mediating ubiquitination and subsequent degradation of LDLR. [UniProt] |
| 细胞定位 | Cytoplasm. Cell membrane; Peripheral membrane protein. [UniProt] |
| 预测分子量 | 50 kDa |
| 翻译后修饰 | Autoubiquitinated. [UniProt] |
检测图片 (1) Click the Picture to Zoom In
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ARG42116 anti-MYLIP / IDOL antibody WB image
Western blot: 25 µg of Jurkat cell lysate stained with ARG42116 anti-MYLIP / IDOL antibody at 1:1000 dilution.
