ARG42171
anti-MYL1 antibody
anti-MYL1 antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes MYL1 |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | FACS, ICC/IF, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | MYL1 |
| 抗原物种 | Human |
| 抗原 | KLH-conjugated synthetic peptide between aa. 101-135 of Human MYL1. |
| 偶联标记 | Un-conjugated |
| 別名 | Myosin light chain A1/A2; MLC1F/MLC3F; MLC1F; MLC1/MLC3; Myosin light chain alkali 1/2; Myosin light chain 1/3, skeletal muscle isoform; MLC3F |
应用说明
| 应用建议 |
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| 应用说明 | IHC-P: Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| 阳性对照 | Human heart | ||||||||||
| 实际分子量 | ~ 25 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein A and immunogen peptide. |
| 缓冲液 | PBS and 0.09% (W/V) Sodium azide. |
| 抗菌剂 | 0.09% (W/V) Sodium azide |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | MYL1 |
| 全名 | myosin, light chain 1, alkali; skeletal, fast |
| 背景介绍 | Myosin is a hexameric ATPase cellular motor protein. It is composed of two heavy chains, two nonphosphorylatable alkali light chains, and two phosphorylatable regulatory light chains. This gene encodes a myosin alkali light chain expressed in fast skeletal muscle. Two transcript variants have been identified for this gene. [provided by RefSeq, Jul 2008] |
| 生物功能 | Non-regulatory myosin light chain required for proper formation and/or maintenance of myofibers, and thus appropriate muscle function. [UniProt] |
| 预测分子量 | 21 kDa |
| 翻译后修饰 | Isoform MLC3 is acetylated at position 2. [UniProt] |
检测图片 (4) Click the Picture to Zoom In
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ARG42171 anti-MYL1 antibody ICC/IF image
Immunofluorescence: 4% Paraformaldehyde-fixed and 0.1% Triton X-100 permeabilized HeLa cells stained with ARG42171 anti-MYL1 antibody (green) at 1:25 dilution. DAPI (blue) for nuclear staining.
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ARG42171 anti-MYL1 antibody IHC-P image
Immunohistochemistry: Paraformaldehyde-fixed and paraffin-embedded Human heart tissue. Tissue was blocked with 3% BSA for 0.5 hour at room temperature. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0). Tissue section was stained with ARG42171 anti-MYL1 antibody at 1:25 dilution for 1 hour at 37°C.
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ARG42171 anti-MYL1 antibody WB image
Western blot: 20 µg of Human heart lysate stained with ARG42171 anti-MYL1 antibody at 1:2000 dilution.
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ARG42171 anti-MYL1 antibody FACS image
Flow Cytometry: HeLa cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. Cells were then incubated in 2% BSA to block non-specific protein-protein interactions followed by ARG42171 anti-MYL1 antibody (right histogram) at 1:25 dilution for 60 min at 37°C, followed by incubation with DyLight®488 labelled secondary antibody. Isotype control antibody (left histogram) was Rabbit IgG1 (1 µg/10^6 cells) used under the same conditions. Acquisition of > 10000 events was performed.
