ARG40447

anti-MYH1 antibody

anti-MYH1 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat

概述

产品描述 Rabbit Polyclonal antibody recognizes MYH1
反应物种 Hu, Ms, Rat
应用 ICC/IF, IHC-P, WB
特异性 The antibody might also react to MYH4 (95%), MYH2 (90%) and MTH8 (85%) based on sequence homology analysis.
宿主 Rabbit
克隆 Polyclonal
同位型 IgG
靶点名称 MYH1
抗原物种 Human
抗原 Recombinant fusion protein corresponding to aa. 1-100 of Human MYH1 (NP_005954.3).
偶联标记 Un-conjugated
別名 Myosin heavy chain IIx/d; MYHa; HEL71; MyHC-IIx/d; Myosin heavy chain 1; MyHC-2x; MyHC-2X/D; Myosin heavy chain, skeletal muscle, adult 1; Myosin heavy chain 2x; Myosin-1; MYHSA1

应用说明

应用建议
应用 推荐稀释比
ICC/IF1:50 - 1:200
IHC-P1:50 - 1:200
WB1:500 - 1:2000
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
阳性对照 Mouse heart
实际分子量 260 kDa

属性

形式 Liquid
纯化 Affinity purified.
缓冲液 PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.
抗菌剂 0.02% Sodium azide
稳定剂 50% Glycerol
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 17879 Mouse MYH1

GeneID: 4619 Human MYH1

Swiss-port # P12882 Human Myosin-1

Swiss-port # Q5SX40 Mouse Myosin-1

基因名称 MYH1
全名 myosin, heavy chain 1, skeletal muscle, adult
背景介绍 Myosin is a major contractile protein which converts chemical energy into mechanical energy through the hydrolysis of ATP. Myosin is a hexameric protein composed of a pair of myosin heavy chains (MYH) and two pairs of nonidentical light chains. Myosin heavy chains are encoded by a multigene family. In mammals at least 10 different myosin heavy chain (MYH) isoforms have been described from striated, smooth, and nonmuscle cells. These isoforms show expression that is spatially and temporally regulated during development. [provided by RefSeq, Jul 2008]
生物功能 Muscle contraction. [UniProt]
细胞定位 Cytoplasm, myofibril. Note=Thick filaments of the myofibrils. [UniProt]
预测分子量 223 kDa

检测图片 (3) Click the Picture to Zoom In

  • ARG40447 anti-MYH1 antibody ICC/IF image

    Immunofluorescence: U2OS cells stained with ARG40447 anti-MYH1 antibody.

  • ARG40447 anti-MYH1 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Rat brain stained with ARG40447 anti-MYH1 antibody at 1:100 dilution.

  • ARG40447 anti-MYH1 antibody WB image

    Western blot: 25 µg of Mouse heart lysate stained with ARG40447 anti-MYH1 antibody at 1:1000 dilution.