ARG66202

anti-MUC16 / CA125 antibody [SQab1724]

anti-MUC16 / CA125 antibody [SQab1724] for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human

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概述

产品描述 Recombinant Rabbit Monoclonal antibody [SQab1724] recognizes MUC16 / CA125
反应物种 Hu
应用 FACS, IHC-P, WB
宿主 Rabbit
克隆 Monoclonal
克隆号 SQab1724
靶点名称 MUC16 / CA125
抗原物种 Human
抗原 Synthetic peptide around aa. 12200‐12300 of MUC16 / CA125.
偶联标记 Un-conjugated
別名 Mucin-16; MUC-16; Ovarian carcinoma antigen CA125; CA125; Ovarian cancer-related tumor marker CA125; CA-125

应用说明

应用建议
应用 推荐稀释比
FACS1:200 - 1:800
IHC-P1:200 ‐ 1:400
WB1:500
应用说明 IHC-P: Antigen retrieval: Heat mediated was performed using Tris/EDTA buffer (pH 9.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式 Liquid
纯化 Affinity purification with immunogen.
缓冲液 PBS, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA.
抗菌剂 0.01% Sodium azide
稳定剂 40% Glycerol and 0.05% BSA
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 94025 Human MUC16

Swiss-port # Q8WXI7 Human Mucin-16

基因名称 MUC16
全名 mucin 16, cell surface associated
生物功能 Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces. [UniProt]
产品亮点 Related products:
MUC16 antibodies; MUC16 ELISA Kits;
Related news:
Cancer Pathology Markers (SQ clones)
预测分子量 1519 kDa
翻译后修饰 Heavily O-glycosylated; expresses both type 1 and type 2 core glycans.
Heavily N-glycosylated; expresses primarily high mannose and complex bisecting type N-linked glycans.
May be phosphorylated. Phosphorylation of the intracellular C-terminal domain may induce proteolytic cleavage and the liberation of the extracellular domain into the extracellular space.
May contain numerous disulfide bridges. Association of several molecules of the secreted form may occur through interchain disulfide bridges providing an extraordinarily large gel-like matrix in the extracellular space or in the lumen of secretory ducts.

检测图片 (4) Click the Picture to Zoom In

  • ARG66202 anti-MUC16 / CA125 antibody [SQab1724] WB image

    Western blot: 30 µg of HeLa and A431 cell lysates stained with ARG66202 anti-MUC16 / CA125 antibody [SQab1724] at 1:500 dilution.

  • ARG66202 anti-MUC16 / CA125 antibody [SQab1724] FACS image

    Flow Cytometry: HeLa cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% TritonX-100 for 15 min. The cells were then stained with ARG66202 anti-MUC16 / CA125 antibody [SQab1724] (red) at 1:800 dilution in 1x PBS/1% BSA for 30 min at room temperature, followed by Alexa Fluor® 488 labelled secondary antibody. Unlabelled sample (black) was used as a control.

  • ARG66202 anti-MUC16 / CA125 antibody [SQab1724] IHC-P image

    Immunohistochemistry: Formalin‐fixed and paraffin‐embedded Human ovarian cancer tissue stained with ARG66202 anti-MUC16 / CA125 antibody [SQab1724] at 1:400 dilution.

    Antigen retrieval: Heat mediated was performed using Tris/EDTA buffer pH 9.0

  • ARG66202 anti-MUC16 / CA125 antibody [SQab1724] IHC-P image

    Immunohistochemistry: Formalin‐fixed and paraffin‐embedded Human ovarian cancer tissue stained with ARG66202 anti-MUC16 / CA125 antibody [SQab1724] at 1:400 dilution.

    Antigen retrieval: Heat mediated was performed using Tris/EDTA buffer pH 9.0

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anti-MUC16 / CA125 antibody [SQab1724]

Application:WB

Sample:HeLa and A431

Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:1:500

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC