ARG42226
anti-MTHFD1L antibody
anti-MTHFD1L antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
概述
| 产品描述 | Goat Polyclonal antibody recognizes MTHFD1L |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 预测物种 | Cow, Dog, Pig |
| 应用 | IHC-P, WB |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | MTHFD1L |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide around the internal region of Human MTHFD1L. (C-DTNDRFLRKITIGQ) (NP_056255.2) |
| 偶联标记 | Un-conjugated |
| 別名 | MTC1THFS; dJ292B18.2; Formyltetrahydrofolate synthetase; EC 6.3.4.3; Monofunctional C1-tetrahydrofolate synthase, mitochondrial; FTHFSDC1 |
应用说明
| 应用建议 |
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| 应用说明 | IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| 实际分子量 | ~ 110 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified |
| 缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q3V3R1 Mouse Monofunctional C1-tetrahydrofolate synthase, mitochondrial Swiss-port # Q6UB35 Human Monofunctional C1-tetrahydrofolate synthase, mitochondrial |
|---|---|
| 基因名称 | MTHFD1L |
| 全名 | methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1-like |
| 背景介绍 | The protein encoded by this gene is involved in the synthesis of tetrahydrofolate (THF) in the mitochondrion. THF is important in the de novo synthesis of purines and thymidylate and in the regeneration of methionine from homocysteine. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2011] |
| 生物功能 | May provide the missing metabolic reaction required to link the mitochondria and the cytoplasm in the mammalian model of one-carbon folate metabolism in embryonic an transformed cells complementing thus the enzymatic activities of MTHFD2. [UniProt] |
| 细胞定位 | Mitochondrion. [UniProt] |
| 预测分子量 | 106 kDa |
检测图片 (3) Click the Picture to Zoom In
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ARG42226 anti-MTHFD1L antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human tonsil tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG42226 anti-MTHFD1L antibody at 5 µg/ml dilution followed by AP-staining.
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ARG42226 anti-MTHFD1L antibody WB image
Western blot: 35 µg of Human cerebellum lysate (in RIPA buffer) stained with ARG42226 anti-MTHFD1L antibody at 0.1 µg/ml dilution and incubated at RT for 1 hour.
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ARG42226 anti-MTHFD1L antibody WB image
Western blot: 35 µg of NIH/3T3, Mouse brain and Rat brain lysates (in RIPA buffer) stained with ARG42226 anti-MTHFD1L antibody at 0.1 µg/ml dilution and incubated at RT for 1 hour.
