ARG45505

anti-MSN / Moesin antibody [8D4]

anti-MSN / Moesin antibody [8D4] for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat,Monkey

概述

产品描述 Mouse Monoclonal antibody [8D4] recognizes MSN / Moesin
反应物种 Hu, Ms, Rat, Mk
应用 FACS, ICC/IF, IHC-P, WB
宿主 Mouse
克隆 Monoclonal
克隆号 8D4
同位型 IgG1
靶点名称 MSN / Moesin
抗原物种 Human
抗原 Recombinant protein containing to human MSN / Moesin.
偶联标记 Un-conjugated
別名 Moesin; Membrane-organizing extension spike protein; HEL70

应用说明

应用建议
应用 推荐稀释比
FACS1 - 3 µg/10^6 cells
ICC/IF5 μg/ml
IHC-P2-5 μg/ml
WB0.25-0.5 μg/ml
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
实际分子量 78 kDa

属性

形式 Liquid
纯化 Affinity purified
缓冲液 0.2% Na2HPO4, 0.9% NaCl and 4% Trehalose.
稳定剂 4% Trehalose
浓度 0.5 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 17698 Mouse MSN

GeneID: 4478 Human MSN

GeneID: 81521 Rat MSN

基因名称 MSN
全名 moesin
背景介绍 Moesin (for membrane-organizing extension spike protein) is a member of the ERM family which includes ezrin and radixin. ERM proteins appear to function as cross-linkers between plasma membranes and actin-based cytoskeletons. Moesin is localized to filopodia and other membranous protrusions that are important for cell-cell recognition and signaling and for cell movement. [provided by RefSeq, Jul 2008]
生物功能 Probably involved in connections of major cytoskeletal structures to the plasma membrane. May inhibit herpes simplex virus 1 infection at an early stage. [UniProt]
细胞定位 Cell membrane; Cell projection; Cytoplasm; Cytoskeleton; Membrane. [UniProt]
预测分子量 68 kDa
翻译后修饰 Phosphorylation on Thr-558 is crucial for the formation of microvilli-like structures. Phosphorylation by ROCK2 suppresses the head-to-tail association of the N-terminal and C-terminal halves resulting in an opened conformation which is capable of actin and membrane-binding (By similarity). Phosphorylation on Thr-558 by STK10 negatively regulates lymphocyte migration and polarization.
S-nitrosylation of Cys-117 is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) implicating the iNOS-S100A8/9 transnitrosylase complex. [UniProt]

检测图片 (6) Click the Picture to Zoom In

  • ARG45505 anti-MSN / Moesin antibody [8D4] ICC/IF image

    Immunofluorescence: SiHa stained with ARG45505 anti-MSN / Moesin antibody [8D4] at 5 μg/ml dilution.

  • ARG45505 anti-MSN / Moesin antibody [8D4] WB image

    Western blot: HL-60 stained with ARG45505 anti-MSN / Moesin antibody [8D4] at 0.5 μg/ml dilution.

  • ARG45505 anti-MSN / Moesin antibody [8D4] FACS image

    Flow Cytometry: U87 stained with ARG45505 anti-MSN / Moesin antibody [8D4] at 1 µg/10^6 cells dilution.

  • ARG45505 anti-MSN / Moesin antibody [8D4] WB image

    Western blot: Rat kidney stained with ARG45505 anti-MSN / Moesin antibody [8D4] at 0.5 μg/ml dilution.

  • ARG45505 anti-MSN / Moesin antibody [8D4] IHC-P image

    Immunohistochemistry: Human gastric adenocarcinoma stained with ARG45505 anti-MSN / Moesin antibody [8D4] at 2 μg/ml dilution.

  • ARG45505 anti-MSN / Moesin antibody [8D4] WB image

    Western blot: COS-7 stained with ARG45505 anti-MSN / Moesin antibody [8D4] at 0.5 μg/ml dilution.