ARG42832
anti-MR1 antibody
anti-MR1 antibody for Flow cytometry,Western blot and Human
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes MR1 |
|---|---|
| 反应物种 | Hu |
| 预测物种 | Ms, Rat |
| 应用 | FACS, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | MR1 |
| 抗原物种 | Human |
| 抗原 | Recombinant protein corresponding to R23-D269 of Human MR1. |
| 偶联标记 | Un-conjugated |
| 別名 | Major histocompatibility complex class I-related gene protein; MHC class I-related gene protein; Class I histocompatibility antigen-like protein; HLALS |
应用说明
| 应用建议 |
|
||||||
|---|---|---|---|---|---|---|---|
| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| 实际分子量 | ~ 39 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | 0.2% Na2HPO4, 0.9% NaCl, 0.05% Sodium azide and 4% Trehalose. |
| 抗菌剂 | 0.05% Sodium azide |
| 稳定剂 | 4% Trehalose |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q95460 Human Major histocompatibility complex class I-related gene protein |
|---|---|
| 基因名称 | MR1 |
| 全名 | major histocompatibility complex, class I-related |
| 背景介绍 | MAIT (mucosal-associated invariant T-cells) lymphocytes represent a small population of T-cells primarily found in the gut. The protein encoded by this gene is an antigen-presenting molecule that presents metabolites of microbial vitamin B to MAITs. This presentation may activate the MAITs to regulate the amounts of specific types of bacteria in the gut. Several transcript variants encoding different isoforms have been found for this gene, and a pseudogene of it has been detected about 36 kbp upstream on the same chromosome. [provided by RefSeq, Jul 2015] |
| 生物功能 | Antigen-presenting molecule specialized in presenting microbial vitamin B metabolites. Involved in the development and expansion of a small population of T-cells expressing an invariant T-cell receptor alpha chain called mucosal-associated invariant T-cells (MAIT). MAIT lymphocytes are preferentially located in the gut lamina propria and therefore may be involved in monitoring commensal flora or serve as a distress signal. Expression and MAIT cell recognition seem to be ligand-dependent. [UniProt] |
| 细胞定位 | Cell membrane; Single-pass membrane protein; Extracellular side. Endoplasmic reticulum. Isoform 4: Secreted. Isoform 3: Cell membrane; Single-pass type I membrane protein. Endoplasmic reticulum membrane. Note=The larger proportion remains in the ER in an immature state. The subset that reach cell surface does it through a B2M-independent pathway. [UniProt] |
| 预测分子量 | 39 kDa |
| 翻译后修饰 | N-glycosylated. [UniProt] |
检测图片 (2) Click the Picture to Zoom In
-
ARG42832 anti-MR1 antibody WB image
Western blot: 50 µg of sample under reducing conditions. T-47D, U-937 and A431 whole cell lysates stained with ARG42832 anti-MR1 antibody at 0.5 µg/ml dilution, overnight at 4°C.
-
ARG42832 anti-MR1 antibody FACS image
Flow Cytometry: SiHa cells were blocked with 10% normal goat serum and then stained with ARG42832 anti-MR1 antibody (blue) at 1 µg/10^6 cells for 30 min at 20°C, followed by incubation with DyLight®488 labelled secondary antibody. Isotype control antibody (green) was Rabbit IgG (1 µg/10^6 cells) used under the same conditions. Unlabelled sample (red) was also used as a control.
