ARG57921
anti-MMP14 / MT1-MMP antibody
anti-MMP14 / MT1-MMP antibody for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes MMP14 / MT1-MMP |
|---|---|
| 反应物种 | Hu |
| 预测物种 | Ms, Rat, Bov, Pig |
| 应用 | FACS, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | MMP14 / MT1-MMP |
| 抗原物种 | Human |
| 抗原 | KLH-conjugated synthetic peptide corresponding to aa. 145-174 (N-terminus) of Human MMP14. |
| 偶联标记 | Un-conjugated |
| 別名 | MT1MMP; MT-MMP 1; Membrane-type matrix metalloproteinase 1; MT1-MMP; Membrane-type-1 matrix metalloproteinase; MT-MMP; EC 3.4.24.80; MMP-X1; MMP-14; Matrix metalloproteinase-14; WNCHRS; MTMMP1 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| 阳性对照 | NCI-H292 |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein G. |
| 缓冲液 | PBS and 0.09% (W/V) Sodium azide. |
| 抗菌剂 | 0.09% (W/V) Sodium azide. |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | MMP14 |
| 全名 | matrix metallopeptidase 14 (membrane-inserted) |
| 背景介绍 | Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. However, the protein encoded by this gene is a member of the membrane-type MMP (MT-MMP) subfamily; each member of this subfamily contains a potential transmembrane domain suggesting that these proteins are expressed at the cell surface rather than secreted. This protein activates MMP2 protein, and this activity may be involved in tumor invasion. [provided by RefSeq, Jul 2008] |
| 生物功能 | Seems to specifically activate progelatinase A. May thus trigger invasion by tumor cells by activating progelatinase A on the tumor cell surface. May be involved in actin cytoskeleton reorganization by cleaving PTK7. Acts as a positive regulator of cell growth and migration via activation of MMP15. Involved in the formation of the fibrovascular tissues in association with pro-MMP2. [UniProt] |
| 细胞定位 | Membrane; Single-pass type I membrane protein. Melanosome. Cytoplasm Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Forms a complex with BST2 and localizes to the cytoplasm. [UniProt] |
| 预测分子量 | 66 kDa |
| 翻译后修饰 | The precursor is cleaved by a furin endopeptidase. Tyrosine phosphorylated by PKDCC/VLK. [UniProt] |
检测图片 (3) Click the Picture to Zoom In
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ARG57921 anti-MMP14 / MT1-MMP antibody IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Human hepatocarcinoma tissue stained with ARG57921 anti-MMP14 / MT1-MMP antibody.
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ARG57921 anti-MMP14 / MT1-MMP antibody WB image
Western blot: 35 µg of NCI-H292 cell lysate stained with ARG57921 anti-MMP14 / MT1-MMP antibody.
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ARG57921 anti-MMP14 / MT1-MMP antibody FACS image
Flow Cytometry: MCF7 cells stained with ARG57921 anti-MMP14 / MT1-MMP antibody (bottom histogram) or without primary antibody as control (top histogram), followed by incubation with FITC labelled secondary antibody.
