ARG66446
anti-MKI67IP / NIFK phospho (Thr234) antibody
anti-MKI67IP / NIFK phospho (Thr234) antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes MKI67IP / NIFK phospho (Thr234) |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | ICC/IF, IHC-P, WB |
| 特异性 | Recognizes endogenous levels of MKI67IP / NIFK phospho (Thr234) protein. |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | MKI67IP / NIFK |
| 抗原物种 | Human |
| 抗原 | KLH-conjugated phosphospecific peptide around Thr234 of Human MKI67IP / NIFK. |
| 偶联标记 | Un-conjugated |
| 別名 | MKI67 FHA domain-interacting nucleolar phosphoprotein; Nucleolar phosphoprotein Nopp34; Nucleolar protein interacting with the FHA domain of pKI-67; Nopp34; MKI67IP; hNIFK |
应用说明
| 应用建议 |
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| 应用说明 | IHC-P: Antigen Retrieval: Heat mediation was performed in Sodium citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| 实际分子量 | ~ 35 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | 0.42% Potassium phosphate (pH 7.3), 0.87% NaCl, 0.01% Sodium azide and 30% Glycerol. |
| 抗菌剂 | 0.01% Sodium azide |
| 稳定剂 | 30% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | NIFK |
| 全名 | nucleolar protein interacting with the FHA domain of MKI67 |
| 背景介绍 | This gene encodes a protein that interacts with the forkhead-associated domain of the Ki-67 antigen. The encoded protein may bind RNA and may play a role in mitosis and cell cycle progression. Multiple pseudogenes exist on chromosomes 5, 10, 12, 15, and 19.[provided by RefSeq, Jan 2009] |
| 细胞定位 | Nucleus, nucleolus. Chromosome. Note=Localizes to mitotic chromosomes in conjunction with MKI67. [UniProt] |
| 预测分子量 | 34 kDa |
| 翻译后修饰 | Sequentially phosphorylated on Thr-238, Thr-234 and Ser-230. Thr-234 is phosphorylated only when Thr-238 is phosphorylated. Likewise, phosphorylation at Ser-230 requires that Thr-234 and Thr-238 are phosphorylated. Phosphorylation enhances MKI67 binding. [UniProt] |
检测图片 (3) Click the Picture to Zoom In
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ARG66446 anti-MKI67IP / NIFK phospho (Thr234) antibody ICC/IF image
Immunofluorescence: Formalin-fixed HeLa cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were stained with ARG66446 anti-MKI67IP / NIFK phospho (Thr234) antibody (red) in 3% BSA-PBS and incubated overnight at 4°C in a hidified chamber.
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ARG66446 anti-MKI67IP / NIFK phospho (Thr234) antibody IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Human brain tissue. Antigen Retrieval: Heat mediation was performed in Sodium citrate buffer (pH 6.0). The section was then stained with ARG66446 anti-MKI67IP / NIFK phospho (Thr234) antibody at RT. The section was counterstained with haematoxylin and mounted with DPX.
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ARG66446 anti-MKI67IP / NIFK phospho (Thr234) antibody WB image
Western blot: HeLa, NIH/3T3 and PC12 whole cell lysates stained with ARG66446 anti-MKI67IP / NIFK phospho (Thr234) antibody.
