ARG56053
anti-MITF antibody [D5]
anti-MITF antibody [D5] for IHC-Formalin-fixed paraffin-embedded sections and Human
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概述
| 产品描述 | Mouse Monoclonal antibody [D5] recognizes MITF |
|---|---|
| 反应物种 | Hu |
| 不反应物种 | Ms, Rat |
| 应用 | IHC-P |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | D5 |
| 同位型 | IgG1, kappa |
| 靶点名称 | MITF |
| 抗原物种 | Human |
| 抗原 | An N-terminus fragment of Human MITF protein |
| 偶联标记 | Un-conjugated |
| 別名 | bHLHe32; Class E basic helix-loop-helix protein 32; MI; Microphthalmia-associated transcription factor; WS2; WS2A; CMM8 |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|
| 应用说明 | IHC-P: Antigen Retrieval: Boil tissue section in 10 mM Citrate buffer (pH 6.0) for 10-20 min, followed by cooling at RT for 20 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein G. |
| 缓冲液 | PBS (pH 7.4), 0.05% Sodium azide and 0.1 mg/ml BSA |
| 抗菌剂 | 0.05% Sodium azide |
| 稳定剂 | 0.1 mg/ml BSA |
| 浓度 | 0.2 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # O75030 Human Microphthalmia-associated transcription factor |
|---|---|
| 基因名称 | MITF |
| 全名 | microphthalmia-associated transcription factor |
| 背景介绍 | This gene encodes a transcription factor that contains both basic helix-loop-helix and leucine zipper structural features. It regulates the differentiation and development of melanocytes retinal pigment epithelium and is also responsible for pigment cell-specific transcription of the melanogenesis enzyme genes. Heterozygous mutations in the this gene cause auditory-pigmentary syndromes, such as Waardenburg syndrome type 2 and Tietz syndrome. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008] |
| 生物功能 | Transcription factor that regulates the expression of genes with essential roles in cell differentiation, proliferation and survival. Binds to symmetrical DNA sequences (E-boxes) (5'-CACGTG-3') found in the promoters of target genes, such as BCL2 and tyrosinase (TYR). Plays an important role in melanocyte development by regulating the expression of tyrosinase (TYR) and tyrosinase-related protein 1 (TYRP1). Plays a critical role in the differentiation of various cell types, such as neural crest-derived melanocytes, mast cells, osteoclasts and optic cup-derived retinal pigment epithelium. [UniProt] |
| 细胞定位 | Nuclear |
| 预测分子量 | 59 kDa |
| 翻译后修饰 | Phosphorylation at Ser-405 significantly enhances the ability to bind the tyrosinase promoter. Phosphorylated at Ser-180 and Ser-516 following KIT signaling, trigerring a short live activation: Phosphorylation at Ser-180 and Ser-516 by MAPK and RPS6KA1, respectively, activate the transcription factor activity but also promote ubiquitination and subsequent degradation by the proteasome. Ubiquitinated following phosphorylation at Ser-180, leading to subsequent degradation by the proteasome. Deubiquitinated by USP13, preventing its degradation. |
检测图片 (1) Click the Picture to Zoom In
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ARG56053 anti-MITF antibody [D5] IHC-P image
Immunohistochemistry: Formalin-fixed, paraffin-embedded Human melanoma stained with ARG56053 anti-MITF antibody [D5].
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anti-MITF antibody [D5]
Application：WB
Sample：HeLa and A549
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：1:100
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
