ARG63504
anti-MID2 / TRIM1 antibody
anti-MID2 / TRIM1 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Gene Regulation antibody; Neuroscience antibody; Signaling Transduction antibody
概述
| 产品描述 | Goat Polyclonal antibody recognizes MID2 / TRIM1 |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 预测物种 | Cow, Dog, Pig |
| 应用 | IHC-P, WB |
| 特异性 | This antibody is expected to recognise both human isoforms of this protein. |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | MID2 / TRIM1 |
| 抗原 | QESPYVSGMKTCH |
| 偶联标记 | Un-conjugated |
| 別名 | RNF60; FXY2; RING finger protein 60; Probable E3 ubiquitin-protein ligase MID2; EC 6.3.2.-; Midline defect 2; MRX101; Midline-2; TRIM1; Tripartite motif-containing protein 1; Midin-2 |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|---|---|
| 应用说明 | IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from goat serum by antigen affinity chromatography. |
| 缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q9QUS6 Mouse Probable E3 ubiquitin-protein ligase MID2 Swiss-port # Q9UJV3 Human Probable E3 ubiquitin-protein ligase MID2 |
|---|---|
| 背景介绍 | The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to microtubular structures in the cytoplasm. Alternate splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq, Feb 2009] |
| 研究领域 | Gene Regulation antibody; Neuroscience antibody; Signaling Transduction antibody |
| 预测分子量 | 83 kDa |
| 翻译后修饰 | Phosphorylated on serine and threonine residues. |
检测图片 (3) Click the Picture to Zoom In
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ARG63504 anti-MID2 / TRIM1 antibody WB image
Western Blot: mouse heart lysate (RIPA buffer, 35 µg total protein per lane) stained with ARG63504 anti-MID2 / TRIM1 antibody at 1 µg/ml dilution.
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ARG63504 anti-MID2 / TRIM1 antibody IHC-P image
Immunohistochemistry: paraffin embedded Human Kidney. (Steamed antigen retrieval with citrate buffer pH 6) stained with ARG63504 anti-MID2 / TRIM1 antibody at 5 µg/ml dilution followed by AP-staining.
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ARG63504 anti-MID2 / TRIM1 antibody WB image
Western blot: 35 µg of Mouse heart (A), Rat heart (B) and Rat kidney (C) lysates (in RIPA buffer) stained with ARG63504 anti-MID2 / TRIM1 antibody at 0.1 µg/ml dilution and incubated at RT for 1 hour.
