ARG45909
anti-MID2 / TRIM1 antibody
anti-MID2 / TRIM1 antibody for Flow cytometry,Western blot and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes MID2 / TRIM1 |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | FACS, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | MID2 / TRIM1 |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide corresponding to middle region of human MID2 / TRIM1. |
| 偶联标记 | Un-conjugated |
| 別名 | Midline 2; RNF60; FXY2; RING finger protein 60; Probable E3 ubiquitin-protein ligase MID2; EC 6.3.2.-; Midline defect 2; MRX101; Midline-2; TRIM1; Tripartite motif-containing protein 1; Midin-2 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| 实际分子量 | 83 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity chromatography purified |
| 缓冲液 | 0.2% Na2HPO4, 0.9% NaCl and 4% Trehalose. |
| 稳定剂 | 4% Trehalose |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -22°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q9QUS6 Mouse Probable E3 ubiquitin-protein ligase MID2 Swiss-port # Q9UJV3 Human Probable E3 ubiquitin-protein ligase MID2 |
|---|---|
| 基因名称 | MID2 |
| 全名 | Midline 2 |
| 背景介绍 | The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to microtubular structures in the cytoplasm. Alternate splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq, Feb 2009] |
| 生物功能 | E3 ubiquitin ligase that plays a role in microtubule stabilization. Mediates the 'Lys-48'-linked polyubiquitination of LRRK2 to drive its localization to microtubules and its proteasomal degradation in neurons. This ubiquitination inhibits LRRK2 kinase activation by RAB29 [UniProt] |
| 细胞定位 | Cytoplasm; Cytoskeleton; Microtubule. [UniProt] |
| 预测分子量 | 83 kDa |
| 翻译后修饰 | Phosphorylated on serine and threonine residues. [UniProt] |
检测图片 (4) Click the Picture to Zoom In
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ARG45909 anti-MID2 / TRIM1 antibody WB image
Western blot: SIHA stained with ARG45909 anti-MID2 / TRIM1 antibody at 0.5 μg/ml dilution.
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ARG45909 anti-MID2 / TRIM1 antibody FACS image
Flow Cytometry: MCF-7 stained with ARG45909 anti-MID2 / TRIM1 antibody at 1 µg/10^6 cells dilution.
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ARG45909 anti-MID2 / TRIM1 antibody WB image
Western blot: Rat lung stained with ARG45909 anti-MID2 / TRIM1 antibody at 0.5 μg/ml dilution.
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ARG45909 anti-MID2 / TRIM1 antibody WB image
Western blot: Mouse lung stained with ARG45909 anti-MID2 / TRIM1 antibody at 0.5 μg/ml dilution.
