ARG55847
anti-MICA antibody
anti-MICA antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes MICA |
|---|---|
| 反应物种 | Hu |
| 应用 | FACS, ICC/IF, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | MICA |
| 抗原物种 | Human |
| 抗原 | KLH-conjugated synthetic peptide corresponding to aa. 68-97 (Center) of Human MICA. |
| 偶联标记 | Un-conjugated |
| 別名 | MHC class I polypeptide-related sequence A; PERB11.1; MIC-A |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||||
| 阳性对照 | Daudi | ||||||||||
| 实际分子量 | ~ 50 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein A and immunogen peptide. |
| 缓冲液 | PBS and 0.09% (W/V) Sodium azide |
| 抗菌剂 | 0.09% (W/V) Sodium azide |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q29983 Human MHC class I polypeptide-related sequence A |
|---|---|
| 基因名称 | MICA |
| 全名 | MHC class I polypeptide-related sequence A |
| 背景介绍 | This gene encodes the highly polymorphic major histocompatability complex class I chain-related protein A. The protein product is expressed on the cell surface, although unlike canonical class I molecules it does not seem to associate with beta-2-microglobulin. It is a ligand for the NKG2-D type II integral membrane protein receptor. The protein functions as a stress-induced antigen that is broadly recognized by intestinal epithelial gamma delta T cells. Variations in this gene have been associated with susceptibility to psoriasis 1 and psoriatic arthritis, and the shedding of MICA-related antibodies and ligands is involved in the progression from monoclonal gammopathy of undetermined significance to multiple myeloma. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jan 2014] |
| 生物功能 | Seems to have no role in antigen presentation. Acts as a stress-induced self-antigen that is recognized by gamma delta T-cells. Ligand for the KLRK1/NKG2D receptor. Binding to KLRK1 leads to cell lysis. [UniProt] |
| 细胞定位 | Cell membrane; Single- pass type I membrane protein Cytoplasm Note=Expressed on the cell surface in gastric epithelium, endothelial cells and fibroblasts and in the cytoplasm in keratinocytes and monocytes. Infection with human adenovirus 5 suppresses cell surface expression due to the adenoviral E3-19K protein which causes retention in the endoplasmic reticulum |
| 预测分子量 | 43 kDa |
| 翻译后修饰 | N-glycosylated. Glycosylation is not essential for interaction with KLRK1/NKG2D but enhances complex formation. Proteolytically cleaved and released from the cell surface of tumor cells which impairs KLRK1/NKG2D expression and T-cell activation. |
检测图片 (4) Click the Picture to Zoom In
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ARG55847 anti-MICA antibody ICC/IF image
Immunofluorescence: MDA-MB-231 cells stained with ARG55847 anti-MICA antibody (green). DAPI (blue) for nuclear staining.
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ARG55847 anti-MICA antibody IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Human hepatocarcinoma stained with ARG55847 anti-MICA antibody.
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ARG55847 anti-MICA antibody WB image
Western blot: 20 µg of Daudi whole cell lysate stained with ARG55847 anti-MICA antibody at 1:1000 dilution.
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ARG55847 anti-MICA antibody FACS image
Flow Cytometry: SK-BR-3 cells stained with ARG55847 anti-MICA antibody (right histogram) at 1:25 dilution or isotype control antibody (left histogram), followed by incubation with Alexa Fluor® 488 labelled secondary antibody.
