ARG43435
anti-MIB1 / Mind bomb 1 antibody
anti-MIB1 / Mind bomb 1 antibody for Western blot and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes MIB1 / Mind bomb 1 |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | MIB1 / Mind bomb 1 |
| 抗原物种 | Human |
| 抗原 | Recombinant fusion protein corresponding to aa. 560-860 of Human MIB1 / Mind bomb 1 (NP_065825.1). |
| 偶联标记 | Un-conjugated |
| 別名 | ZZZ6; DAPK-interacting protein 1; DIP-1; EC 6.3.2.-; E3 ubiquitin-protein ligase MIB1; DIP1; Mind bomb homolog 1; MIB; Zinc finger ZZ type with ankyrin repeat domain protein 2; LVNC7; ZZANK2 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||
| 阳性对照 | A549 | ||||
| 实际分子量 | ~ 110 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified. |
| 缓冲液 | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | MIB1 |
| 全名 | mindbomb E3 ubiquitin protein ligase 1 |
| 背景介绍 | This gene encodes a protein containing multiple ankyrin repeats and RING finger domains that functions as an E3 ubiquitin ligase. The encoded protein positively regulates Notch signaling by ubiquitinating the Notch receptors, thereby facilitating their endocytosis. This protein may also promote the ubiquitination and degradation of death-associated protein kinase 1 (DAPK1). [provided by RefSeq, Jun 2013] |
| 生物功能 | E3 ubiquitin-protein ligase that mediates ubiquitination of Delta receptors, which act as ligands of Notch proteins. Positively regulates the Delta-mediated Notch signaling by ubiquitinating the intracellular domain of Delta, leading to endocytosis of Delta receptors. Probably mediates ubiquitination and subsequent proteasomal degradation of DAPK1, thereby antagonizing anti-apoptotic effects of DAPK1 to promote TNF-induced apoptosis (By similarity). Involved in ubiquitination of centriolar satellite CEP131, CEP290 and PCM1 proteins and hence inhibits primary cilium formation in proliferating cells. Mediates 'Lys-63'-linked polyubiquitination of TBK1, which probably participates in kinase activation. [UniProt] |
| 细胞定位 | Cytoplasm. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriolar satellite. Cell membrane. Note=Localizes to the plasma membrane (By similarity). According to PubMed:15048887, it is mitochondrial, however such localization remains unclear. Displaced from centriolar satellites in response to cellular stress, such as ultraviolet light (UV) radiation or heat shock. [UniProt] |
| 预测分子量 | 110 kDa |
| 翻译后修饰 | Ubiquitinated; possibly via autoubiquitination (By similarity). Ubiquitinated; this modification is inhibited in response to cellular stress, such as ultraviolet light (UV) radiation or heat shock. [UniProt] |
检测图片 (1) Click the Picture to Zoom In
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ARG43435 anti-MIB1 / Mind bomb 1 antibody WB image
Western blot: 25 µg of A549 cell lysate stained with ARG43435 anti-MIB1 / Mind bomb 1 antibody at 1:1000 dilution.
