ARG67040

anti-MHC class I antibody [SQab30331]

anti-MHC class I antibody [SQab30331] for IHC-Formalin-fixed paraffin-embedded sections and Human

概述

产品描述 Recombinant rabbit Monoclonal antibody [SQab30331] recognizes HLA
反应物种 Hu
应用 IHC-P
宿主 Rabbit
克隆 Monoclonal
克隆号 SQab30331
同位型 IgG
靶点名称 HLA
抗原物种 Human
抗原 Recombinant protein of Human MHC class I.
偶联标记 Un-conjugated
Protein Full name HLA class I histocompatibility antigen
別名 HLA-A, Major Histocompatibility Complex, Class I, A, HLA Class I Histocompatibility Antigen, A Alpha Chain, HLAA, HLA Class I Histocompatibility Antigen, A-1 Alpha Chain, MHC Class I Antigen HLA-A Heavy Chain, Leukocyte Antigen Class I-A, Human Leukocyte Antigen A, HLA-B 2, Major Histocompatibility Complex, Class I, B, HLA Class I Histocompatibility Antigen, B Alpha Chain, HLAB, AS, MHC Class I Antigen HLA-B Alpha Chain, MHC Class I Antigen HLA-B Heavy Chain , MHC HLA-B Transmembrane Glycoprotein, MHC HLA-B Cell Surface Glycoprotein, HLA-C, Major Histocompatibility Complex, Class I, C, HLA Class I Histocompatibility Antigen, C Alpha Chain, HLA-JY3, D6S204, PSORS1, HLAC

应用说明

应用建议
应用 推荐稀释比
IHC-P1:50 - 1:100
应用说明 The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
阳性对照 Tonsil

属性

形式 Liquid
纯化 Purification with Protein A.
缓冲液 PBS, 0.01% Sodium azide, 40% Glycerol and 0.05%BSA.
抗菌剂 0.01% Sodium azide
稳定剂 40% Glycerol and 0.05%BSA
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

基因名称 HLA-A, HLA-B, HLA-C
全名 Major Histocompatibility Complex, Class I, A; Major Histocompatibility Complex, Class I, B; Major Histocompatibility Complex, Class I, C
背景介绍 HLA-A belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen so that they can be recognized by cytotoxic T cells. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon 1 encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. More than 6000 HLA-A alleles have been described. The HLA system plays an important role in the occurrence and outcome of infectious diseases, including those caused by the malaria parasite, the human immunodeficiency virus (HIV), and the severe acute respiratory syndrome coronavirus (SARS-CoV). The structural spike and the nucleocapsid proteins of the novel coronavirus SARS-CoV-2, which causes coronavirus disease 2019 (COVID-19), are reported to contain multiple Class I epitopes with predicted HLA restrictions. Individual HLA genetic variation may help explain different immune responses to a virus across a population.[provided by RefSeq, Aug 2020]
生物功能 Allele A*26:01: Presents several epitopes derived from HIV-1 gag-pol (EVIPMFSAL, ETKLGKAGY) and env (LVSDGGPNLY), carrying as anchor residues preferentially Glu at position 1, Val or Thr at position 2 and Tyr at the C-terminus. Allele A*29:02: Presents peptides having a common motif, namely a Glu residue at position 2 and Tyr or Leu anchor residues at the C-terminus. Allele A*32:01: Interacts with natural killer (NK) cell receptor KIR3DL1 and may contribute to functional maturation of NK cells and self-nonself discrimination during innate immune response. Allele A*68:01: Presents viral epitopes derived from IAV NP (KTGGPIYKR) and HIV-1 tat (ITKGLGISYGR), having a common signature motif namely, Val or Thr at position 2 and positively charged residues Arg or Lys at the C-terminal anchor. [UniProt]
细胞定位 Cell membrane, Endoplasmic reticulum, Membrane, MHC I
预测分子量 40 kDa
翻译后修饰 Polyubiquitinated in a post ER compartment by interaction with human herpesvirus 8 MIR1 protein. This targets the protein for rapid degradation via the ubiquitin system. N-linked glycosylation at Asn-110. [UniProt]

检测图片 (1) Click the Picture to Zoom In

  • ARG67040 anti-MHC class I antibody [SQab30331] IHC-P image

    Immunohistochemistry: Formalin-fixed and paraffin-embedded tonsil stained with ARG67040 anti-MHC class I antibody [SQab30331].