anti-MGMT antibody [SQab1861]

anti-MGMT antibody [SQab1861] for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human


产品描述 Recombinant Rabbit Monoclonal antibody [SQab1861] recognizes MGMT
反应物种 Hu
宿主 Rabbit
克隆 Monoclonal
克隆号 SQab1861
同位型 IgG
靶点名称 MGMT
抗原物种 Human
抗原 Synthetic peptide around the N-terminus of Human MGMT.
偶联标记 Un-conjugated
別名 O-6-methylguanine-DNA-alkyltransferase; Methylated-DNA--protein-cysteine methyltransferase; MGMT; EC; 6-O-methylguanine-DNA methyltransferase


应用 推荐稀释比
FACS1:10 - 1:200
IHC-P1:100 - 1:200
WB1:5000 - 1:10000
应用说明 IHC-P: Antigen Retrieval: Heat mediated was performed using Tris/EDTA buffer (pH 9.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.


形式 Liquid
纯化 Purification with Protein A.
缓冲液 PBS, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA.
抗菌剂 0.01% Sodium azide
稳定剂 40% Glycerol and 0.05% BSA
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.



GeneID: 4255 Human MGMT

Swiss-port # P16455 Human Methylated-DNA--protein-cysteine methyltransferase

基因名称 MGMT
全名 O-6-methylguanine-DNA methyltransferase
背景介绍 Alkylating agents are potent carcinogens that can result in cell death, mutation and cancer. MGMT is a DNA repair protein that is involved in cellular defense against mutagenesis and toxicity from alkylating agents. The protein catalyzes transfer of methyl groups from O(6)-alkylguanine and other methylated moieties of the DNA to its own molecule, which repairs the toxic lesions. Methylation of the genes promoter has been associated with several cancer types, including colorectal cancer, lung cancer, lymphoma and glioblastoma. [provided by RefSeq, Sep 2015]
生物功能 MGMT involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) and O4-methylthymine (O4-MeT) in DNA. Repairs the methylated nucleobase in DNA by stoichiometrically transferring the methyl group to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated. [UniProt]
产品亮点 Related products:
MGMT antibodies; Anti-Rabbit IgG secondary antibodies;
Related news:
Cancer Pathology Markers (SQ clones)
预测分子量 22 kDa

检测图片 (4) Click the Picture to Zoom In

  • ARG66313 anti-MGMT antibody [SQab1861] FACS image

    Flow Cytometry: Jurkat cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% TritonX-100 for 15 min. The cells were stained with ARG66313 anti-MGMT antibody [SQab1861] at 1:50 dilution (blue) in 1x PBS/1% BSA for 30 min at 4°C, followed by Alexa Fluor® 488 labelled secondary antibody. Unlabelled sample (red) was used as a control. .

  • ARG66313 anti-MGMT antibody [SQab1861] IHC-P image

    Immunohistochemistry: Formalin-fixed and paraffin-embedded Human breast tissue stained with ARG66313 anti-MGMT antibody [SQab1861] at 1:200 dilution. Antigen Retrieval: Heat mediated was performed using Tris/EDTA buffer pH 9.0.

  • ARG66313 anti-MGMT antibody [SQab1861] WB image

    Western blot: 20 µg of MCF7, Jurkat, HeLa and HT-29 cell lysates stained with ARG66313 anti-MGMT antibody [SQab1861] at 1:5000 dilution.

  • ARG66313 anti-MGMT antibody [SQab1861] IP image

    Immunoprecipitation: 0.4 mg of HeLa whole cell lysate was immunoprecipitated (1:80 dilution) and stained with ARG66313 anti-MGMT antibody [SQab1861].

    Lane 1: Immunoprecipitation in HeLa whole cell lysate
    Lane 2: PBS instead of Primary Ab in HeLa whole cell lysate
    Lane 3: HeLa whole cell lysate, 10 µg (input)