ARG56406
anti-METTL3 antibody
anti-METTL3 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
参考文献1
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes METTL3 |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | ICC/IF, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | METTL3 |
| 抗原物种 | Human |
| 抗原 | Recombinant protein of Human METTL3 |
| 偶联标记 | Un-conjugated |
| 別名 | Spo8; IME4; Methyltransferase-like protein 3; EC 2.1.1.62; N6-adenosine-methyltransferase 70 kDa subunit; M6A; MT-A70 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| 抗菌剂 | 0.02% sodium azide |
| 稳定剂 | 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q86U44 Human N6-adenosine-methyltransferase 70 kDa subunit Swiss-port # Q8C3P7 Mouse N6-adenosine-methyltransferase subunit METTL3 |
|---|---|
| 基因名称 | METTL3 |
| 全名 | methyltransferase like 3 |
| 背景介绍 | This gene encodes the 70 kDa subunit of MT-A which is part of N6-adenosine-methyltransferase. This enzyme is involved in the posttranscriptional methylation of internal adenosine residues in eukaryotic mRNAs, forming N6-methyladenosine. [provided by RefSeq, Jul 2008] |
| 生物功能 | N6-methyltransferase that methylates adenosine residues of some RNAs and acts as a regulator of the circadian clock, differentiation of embryonic stem cells and primary miRNA processing. N6-methyladenosine (m6A), which takes place at the 5'-[AG]GAC-3' consensus sites of some mRNAs, plays a role in the efficiency of mRNA splicing, processing, editing and mRNA stability. M6A regulates the length of the circadian clock: acts as a early pace-setter in the circadian loop by putting mRNA production on a fast-track for facilitating nuclear processing, thereby providing an early point of control in setting the dynamics of the feedback loop (By similarity). M6A also acts as a regulator of mRNA stability: in embryonic stem cells (ESCs), m6A methylation of mRNAs encoding key naive pluripotency-promoting transcripts results in transcript destabilization, promoting differentiation of ESCs (By similarity). M6A also takes place in other RNA molecules, such as primary miRNA (pri-miRNAs). Mediates methylation of pri-miRNAs, marking them for recognition and processing by DGCR8. [UniProt] |
| 预测分子量 | 64 kDa |
检测图片 (4) Click the Picture to Zoom In
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ARG56406 anti-METTL3 antibody WB image
Western blot: Mouse kidney stained with ARG56406 anti-METTL3 antibody.
From Liu B et al. Ann Transl Med. (2022), doi: 10.21037/atm-22-845, Fig. 6. B.
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ARG56406 anti-METTL3 antibody WB image
Western blot: 30 µg of 293T, COS-7 and Mouse testis lysates stained with ARG56406 anti-METTL3 antibody at 1:500 dilution.
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ARG56406 anti-METTL3 antibody ICC/IF image
Immunofluorescence: C6 cells stained with ARG56406 anti-METTL3 antibody.
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ARG56406 anti-METTL3 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human stomach stained with ARG56406 anti-METTL3 antibody at 1:100 dilution.
客户反馈
Excellent
anti-METTL3 antibody
Application：WB
Sample：HepG2
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：1:500
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
文献引用
Transcriptomic analysis and laboratory experiments reveal potential critical genes and regulatory mechanisms in sepsis-associated acute kidney injury
WB / Mouse
