ARG10816
anti-MERTK phospho (Tyr749 / Tyr753 / Tyr754) antibody
anti-MERTK phospho (Tyr749 / Tyr753 / Tyr754) antibody for Confocal microscopy (Confocal),Dot blot,ELISA,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat,Monkey
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes MERTK phospho (Tyr749 / Tyr753 / Tyr754) |
|---|---|
| 反应物种 | Hu, Ms, Rat, Mk |
| 应用 | Confocal, Dot, ELISA, ICC/IF, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | MERTK |
| 抗原物种 | Human |
| 抗原 | Phosphospecific peptide corresponding to aa. 746-757 of Human MERTK. (CKKI-Y(p)-SGD-Y(p)-Y(p)-RQG) |
| 偶联标记 | Un-conjugated |
| 別名 | c-mer; Receptor tyrosine kinase MerTK; c-Eyk; Tyro12; MER; RP38; EC 2.7.10.1; Proto-oncogene c-Mer; Tyrosine-protein kinase Mer |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||||||||
| 实际分子量 | ~ 175 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified. |
| 缓冲液 | Tris-Glycine Buffer (pH 7.4 - 7.8), Hepes, 0.02% Sodium azide, 30% Glycerol and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 30% Glycerol and 0.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | MERTK |
| 全名 | MER proto-oncogene, tyrosine kinase |
| 背景介绍 | This gene is a member of the MER/AXL/TYRO3 receptor kinase family and encodes a transmembrane protein with two fibronectin type-III domains, two Ig-like C2-type (immunoglobulin-like) domains, and one tyrosine kinase domain. Mutations in this gene have been associated with disruption of the retinal pigment epithelium (RPE) phagocytosis pathway and onset of autosomal recessive retinitis pigmentosa (RP). [provided by RefSeq, Jul 2008] |
| 生物功能 | Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to several ligands including LGALS3, TUB, TULP1 or GAS6. Regulates many physiological processes including cell survival, migration, differentiation, and phagocytosis of apoptotic cells (efferocytosis). Ligand binding at the cell surface induces autophosphorylation of MERTK on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, interacts with GRB2 or PLCG2 and induces phosphorylation of MAPK1, MAPK2, FAK/PTK2 or RAC1. MERTK signaling plays a role in various processes such as macrophage clearance of apoptotic cells, platelet aggregation, cytoskeleton reorganization and engulfment. Functions in the retinal pigment epithelium (RPE) as a regulator of rod outer segments fragments phagocytosis. Plays also an important role in inhibition of Toll-like receptors (TLRs)-mediated innate immune response by activating STAT1, which selectively induces production of suppressors of cytokine signaling SOCS1 and SOCS3. [UniProt] |
| 预测分子量 | 110 kDa |
| 翻译后修饰 | Autophosphorylated on Tyr-749, Tyr-753 and Tyr-754 in the activation loop allowing full activity. Autophosphorylated on Tyr-872 leading to recruitment of downstream partners of the signaling cascade such as PLCG2 (By similarity). |
检测图片 (1) Click the Picture to Zoom In
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ARG10816 anti-MERTK phospho (Tyr749 / Tyr753 / Tyr754) antibody WB image
Western blot: 1) Phosphorylated, and 2,3) non-phosphorylated MERTK was immunoprecipitated with ARG10784 anti-MERTK antibody and stained with ARG10816 anti-MERTK phospho (Tyr749 / Tyr753 / Tyr754) antibody. MW of MERTK is 174 kDa. Lower broad band is IgG from immunoprecipitation.
