ARG44230
anti-MELK antibody
anti-MELK antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes MELK |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | FACS, ICC/IF, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | MELK |
| 抗原物种 | Human |
| 抗原 | Recombinant protein of Human MELK |
| 偶联标记 | Un-conjugated |
| Protein Full name | Maternal embryonic leucine zipper kinase |
| 別名 | MELK; Maternal Embryonic Leucine Zipper Kinase; KIAA0175; Tyrosine-Protein Kinase MELK; Protein Kinase PK38; Protein Kinase Eg3; PEg3 Kinase; EC 2.7.11.1; HPK38; EC 2.7.10.2; EC 2.7.11; HMELK |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | 0.9% NaCl, 0.2% Na2HPO4, 0.05% Sodium azide and 5% BSA. |
| 抗菌剂 | 0.05% Sodium azide |
| 稳定剂 | 5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q14680 Human Maternal embryonic leucine zipper kinase Swiss-port # Q61846 Mouse Maternal embryonic leucine zipper kinase |
|---|---|
| 基因名称 | MELK |
| 全名 | Maternal Embryonic Leucine Zipper Kinase |
| 背景介绍 | Enables calcium ion binding activity; non-membrane spanning protein tyrosine kinase activity; and protein serine/threonine kinase activity. Involved in apoptotic process; cell population proliferation; and protein autophosphorylation. Located in cell cortex and plasma membrane. |
| 生物功能 | Serine/threonine-protein kinase involved in various processes such as cell cycle regulation, self-renewal of stem cells, apoptosis and splicing regulation. Has a broad substrate specificity; phosphorylates BCL2L14, CDC25B, MAP3K5/ASK1 and ZNF622. Acts as an activator of apoptosis by phosphorylating and activating MAP3K5/ASK1. Acts as a regulator of cell cycle, notably by mediating phosphorylation of CDC25B, promoting localization of CDC25B to the centrosome and the spindle poles during mitosis. Plays a key role in cell proliferation and carcinogenesis. Required for proliferation of embryonic and postnatal multipotent neural progenitors. Phosphorylates and inhibits BCL2L14, possibly leading to affect mammary carcinogenesis by mediating inhibition of the pro-apoptotic function of BCL2L14. Also involved in the inhibition of spliceosome assembly during mitosis by phosphorylating ZNF622, thereby contributing to its redirection to the nucleus. May also play a role in primitive hematopoiesis. |
| 细胞定位 | Cell membrane, Membrane |
| 预测分子量 | 75 kDa |
| 翻译后修饰 | Phosphoprotein |
检测图片 (4) Click the Picture to Zoom In
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ARG44230 anti-MELK antibody ICC/IF image
Immunofluorescence: A549 stained with ARG44230 anti-MELK antibody at 5 μg/ml dilution.
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ARG44230 anti-MELK antibody WB image
Western blot: K562, U87 and Jurkat stained with ARG44230 anti-MELK antibody at 0.5 μg/mL dilution.
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ARG44230 anti-MELK antibody IHC-P image
Immunohistochemistry: Rat ovary stained with ARG44230 anti-MELK antibody at 2 μg/ml dilution.
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ARG44230 anti-MELK antibody IHC-P image
Immunohistochemistry: Mouse ovary stained with ARG44230 anti-MELK antibody at 2 μg/ml dilution.
