ARG52336
anti-MEK5 phospho (Ser311 / Thr315) antibody
anti-MEK5 phospho (Ser311 / Thr315) antibody for Western blot and Rat
Cancer antibody; Cell Biology and Cellular Response antibody; Signaling Transduction antibody
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes MEK5 phospho (Ser311 / Thr315) |
|---|---|
| 反应物种 | Rat |
| 预测物种 | Hu, Ms, Bov, Chk, Dog, NHuPrm, Xenopus laevis, Zfsh |
| 应用 | WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | MEK5 |
| 抗原物种 | Rat |
| 抗原 | Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser311/Thr315 conjugated to KLH |
| 偶联标记 | Un-conjugated |
| 別名 | MEK 5; HsT17454; MAPK/ERK kinase 5; PRKMK5; EC 2.7.12.2; MAPKK 5; MEK5; MAPKK5; Dual specificity mitogen-activated protein kinase kinase 5; MAP kinase kinase 5 |
应用说明
| 应用建议 |
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| 应用说明 | Specific for the ~49k MEK5 protein phosphorylated at Ser311, Thr315. Immunolabeling of the MEK5 band is blocked by preadsorption with the phospho-peptide used as antigen but not be the corresponding dephospho-peptide. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity Purified |
| 缓冲液 | 10 mM HEPES (pH 7.5), 150 mM NaCl, 0.1 mg/ml BSA and 50% Glycerol |
| 稳定剂 | 0.1 mg/ml BSA, 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q62862 Rat Dual specificity mitogen-activated protein kinase kinase 5 |
|---|---|
| 基因名称 | MAP2K5 |
| 全名 | mitogen activated protein kinase kinase 5 |
| 背景介绍 | MEK5 (also known as MKK5) is a dual specificity serine/threonine protein kinase belonging to the MAP kinase kinase family. MEK5 has been shown to specifically activate ERK5 (Zhou et al., 1995) whereas MEK5 itself is regulated by MEKK3 (Chao et al., 1999). An important link between MEK5 and metastatic prostrate cancer has been demonstrated (Mehta et.al., 2003). Dual phosphorylation of Ser311 and Thr315 have been implicated in cell proliferation (Cameron et al., 2004). |
| 研究领域 | Cancer antibody; Cell Biology and Cellular Response antibody; Signaling Transduction antibody |
| 预测分子量 | 50 kDa |
| 翻译后修饰 | Activated by phosphorylation on Ser/Thr by MAP kinase kinase kinases. Yersinia yopJ may acetylate Ser/Thr residues, preventing phosphorylation and activation, thus blocking the MAPK signaling pathway. |
检测图片 (1) Click the Picture to Zoom In
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ARG52336 anti-MEK5 phospho (Ser311 / Thr315) antibody WB image
Western blot: Rat testis lysate showing phospho-specific immunolabeling of the ~49 kDa MEK5 protein phosphorylated at Ser311 and Thr315 stained with ARG52336 anti-MEK5 phospho (Ser311 / Thr315) antibody.
