ARG40804
anti-MEK5 antibody
anti-MEK5 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes MEK5 |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | ICC/IF, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | MEK5 |
| 抗原物种 | Human |
| 抗原 | Recombinant fusion protein corresponding to aa. 1-190 of Human MEK5 (NP_660143.1). |
| 偶联标记 | Un-conjugated |
| 別名 | MEK 5; HsT17454; MAPK/ERK kinase 5; PRKMK5; EC 2.7.12.2; MAPKK 5; MEK5; MAPKK5; Dual specificity mitogen-activated protein kinase kinase 5; MAP kinase kinase 5 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| 阳性对照 | BT-474 | ||||||||
| 实际分子量 | ~ 50 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified. |
| 缓冲液 | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | MAP2K5 |
| 全名 | mitogen-activated protein kinase kinase 5 |
| 背景介绍 | The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase specifically interacts with and activates MAPK7/ERK5. This kinase itself can be phosphorylated and activated by MAP3K3/MEKK3, as well as by atypical protein kinase C isoforms (aPKCs). The signal cascade mediated by this kinase is involved in growth factor stimulated cell proliferation and muscle cell differentiation. Three alternatively spliced transcript variants of this gene encoding distinct isoforms have been described. [provided by RefSeq, May 2011] |
| 生物功能 | Acts as a scaffold for the formation of a ternary MAP3K2/MAP3K3-MAP3K5-MAPK7 signaling complex. Activation of this pathway appears to play a critical role in protecting cells from stress-induced apoptosis, neuronal survival and cardiac development and angiogenesis. [UniProt] |
| 预测分子量 | 50 kDa |
| 翻译后修饰 | Activated by phosphorylation on Ser/Thr by MAP kinase kinase kinases. Yersinia yopJ may acetylate Ser/Thr residues, preventing phosphorylation and activation, thus blocking the MAPK signaling pathway. [UniProt] |
检测图片 (3) Click the Picture to Zoom In
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ARG40804 anti-MEK5 antibody ICC/IF image
Immunofluorescence: MCF7 cells stained with ARG40804 anti-MEK5 antibody.
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ARG40804 anti-MEK5 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Rat brain stained with ARG40804 anti-MEK5 antibody at 1:100 dilution.
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ARG40804 anti-MEK5 antibody WB image
Western blot: 25 µg of BT-474 cell lysate stained with ARG40804 anti-MEK5 antibody at 1:500 dilution.
