ARG51644
anti-MEK3 / MKK3 phospho (Ser189) antibody
anti-MEK3 / MKK3 phospho (Ser189) antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Cancer antibody; Gene Regulation antibody; Immune System antibody; Signaling Transduction antibody
概述
产品描述 | Rabbit Polyclonal antibody recognizes MEK3 / MKK3 phospho (Ser189) |
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反应物种 | Hu, Ms, Rat |
应用 | ICC/IF, IHC-P, WB |
宿主 | Rabbit |
克隆 | Polyclonal |
同位型 | IgG |
靶点名称 | MEK3 / MKK3 |
抗原物种 | Human |
抗原 | Peptide sequence around phosphorylation site of serine 189 (V-D-S(p)-V-A) derived from Human MKK3 / MEK3. |
偶联标记 | Un-conjugated |
別名 | SAPK kinase 2; MEK 3; MAPKK 3; Stress-activated protein kinase kinase 2; PRKMK3; EC 2.7.12.2; MAPK/ERK kinase 3; MEK3; MAPKK3; SAPKK2; SAPKK-2; MAP kinase kinase 3; MKK3; Dual specificity mitogen-activated protein kinase kinase 3 |
应用说明
应用建议 |
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应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
形式 | Liquid |
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纯化 | Antibodies were produced by immunizing rabbits with KLH-conjugated synthetic phosphopeptide. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. In addition, non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
缓冲液 | PBS (without Mg2+ and Ca2+, pH 7.4), 150mM NaCl, 0.02% Sodium azide and 50% Glycerol. |
抗菌剂 | 0.02% Sodium azide |
稳定剂 | 50% Glycerol |
浓度 | 1 mg/ml |
存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
数据库连接 |
Swiss-port # O09110 Mouse Dual specificity mitogen-activated protein kinase kinase 3 Swiss-port # P46734 Human Dual specificity mitogen-activated protein kinase kinase 3 |
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基因名称 | MAP2K3 |
全名 | mitogen-activated protein kinase kinase 3 |
背景介绍 | Dual specificity kinase. Is activated by cytokines and environmental stress in vivo. Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in the MAP kinase p38. |
生物功能 | Dual specificity kinase. Is activated by cytokines and environmental stress in vivo. Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in the MAP kinase p38. [UniProt] |
研究领域 | Cancer antibody; Gene Regulation antibody; Immune System antibody; Signaling Transduction antibody |
预测分子量 | 39 kDa |
翻译后修饰 | Autophosphorylated. Phosphorylation on Ser-218 and Thr-222 by MAP kinase kinase kinases regulates positively the kinase activity (PubMed:8622669). Phosphorylated by TAOK2 (PubMed:11279118). Yersinia yopJ may acetylate Ser/Thr residues, preventing phosphorylation and activation, thus blocking the MAPK signaling pathway. |
检测图片 (4) Click the Picture to Zoom In
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ARG51644 anti-MEK3 / MKK3 phospho (Ser189) antibody WB image
Western blot: Extracts from HeLa cells untreated or treated with PMA stained with ARG51644 anti-MEK3 / MKK3 phospho (Ser189) antibody.
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ARG51644 anti-MEK3 / MKK3 phospho (Ser189) antibody WB image
Western blot: Extracts from 3T3 cells, treated with Anisomycin or calf intestinal phosphatase (CIP), stained with ARG51644 anti-MEK3 / MKK3 phospho (Ser189) antibody.
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ARG51644 anti-MEK3 / MKK3 phospho (Ser189) antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human breast carcinoma tissue stained with ARG51644 anti-MEK3 / MKK3 phospho (Ser189) antibody (left) or the same antibody preincubated with blocking peptide (right).
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ARG51644 anti-MEK3 / MKK3 phospho (Ser189) antibody ICC/IF image
Immunofluorescence: Methanol-fixed HeLa cells stained with ARG51644 anti-MEK3 / MKK3 phospho (Ser189) antibody.