ARG51644

anti-MEK3 / MKK3 phospho (Ser189) antibody

anti-MEK3 / MKK3 phospho (Ser189) antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat

Cancer antibody; Gene Regulation antibody; Immune System antibody; Signaling Transduction antibody

概述

产品描述 Rabbit Polyclonal antibody recognizes MEK3 / MKK3 phospho (Ser189)
反应物种 Hu, Ms, Rat
应用 ICC/IF, IHC-P, WB
宿主 Rabbit
克隆 Polyclonal
同位型 IgG
靶点名称 MEK3 / MKK3
抗原物种 Human
抗原 Peptide sequence around phosphorylation site of serine 189 (V-D-S(p)-V-A) derived from Human MKK3 / MEK3.
偶联标记 Un-conjugated
別名 SAPK kinase 2; MEK 3; MAPKK 3; Stress-activated protein kinase kinase 2; PRKMK3; EC 2.7.12.2; MAPK/ERK kinase 3; MEK3; MAPKK3; SAPKK2; SAPKK-2; MAP kinase kinase 3; MKK3; Dual specificity mitogen-activated protein kinase kinase 3

应用说明

应用建议
应用 推荐稀释比
ICC/IF1:100 - 1:200
IHC-P1:50 - 1:100
WB1:500 - 1:1000
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式 Liquid
纯化 Antibodies were produced by immunizing rabbits with KLH-conjugated synthetic phosphopeptide. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. In addition, non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
缓冲液 PBS (without Mg2+ and Ca2+, pH 7.4), 150mM NaCl, 0.02% Sodium azide and 50% Glycerol.
抗菌剂 0.02% Sodium azide
稳定剂 50% Glycerol
浓度 1 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 26397 Mouse MAP2K3

GeneID: 5606 Human MAP2K3

Swiss-port # O09110 Mouse Dual specificity mitogen-activated protein kinase kinase 3

Swiss-port # P46734 Human Dual specificity mitogen-activated protein kinase kinase 3

基因名称 MAP2K3
全名 mitogen-activated protein kinase kinase 3
背景介绍 Dual specificity kinase. Is activated by cytokines and environmental stress in vivo. Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in the MAP kinase p38.
生物功能 Dual specificity kinase. Is activated by cytokines and environmental stress in vivo. Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in the MAP kinase p38. [UniProt]
研究领域 Cancer antibody; Gene Regulation antibody; Immune System antibody; Signaling Transduction antibody
预测分子量 39 kDa
翻译后修饰 Autophosphorylated. Phosphorylation on Ser-218 and Thr-222 by MAP kinase kinase kinases regulates positively the kinase activity (PubMed:8622669). Phosphorylated by TAOK2 (PubMed:11279118).
Yersinia yopJ may acetylate Ser/Thr residues, preventing phosphorylation and activation, thus blocking the MAPK signaling pathway.

检测图片 (4) Click the Picture to Zoom In

  • ARG51644 anti-MEK3 / MKK3 phospho (Ser189) antibody WB image

    Western blot: Extracts from HeLa cells untreated or treated with PMA stained with ARG51644 anti-MEK3 / MKK3 phospho (Ser189) antibody.

  • ARG51644 anti-MEK3 / MKK3 phospho (Ser189) antibody WB image

    Western blot: Extracts from 3T3 cells, treated with Anisomycin or calf intestinal phosphatase (CIP), stained with ARG51644 anti-MEK3 / MKK3 phospho (Ser189) antibody.

  • ARG51644 anti-MEK3 / MKK3 phospho (Ser189) antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human breast carcinoma tissue stained with ARG51644 anti-MEK3 / MKK3 phospho (Ser189) antibody (left) or the same antibody preincubated with blocking peptide (right).

  • ARG51644 anti-MEK3 / MKK3 phospho (Ser189) antibody ICC/IF image

    Immunofluorescence: Methanol-fixed HeLa cells stained with ARG51644 anti-MEK3 / MKK3 phospho (Ser189) antibody.