ARG51108
anti-MEK3 / MKK3 antibody
anti-MEK3 / MKK3 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Cancer antibody; Gene Regulation antibody; Immune System antibody; Signaling Transduction antibody
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes MEK3 / MKK3 |
|---|---|
| 反应物种 | Hu |
| 预测物种 | Ms, Rat |
| 应用 | ICC/IF, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | MEK3 / MKK3 |
| 抗原物种 | Human |
| 抗原 | Peptide sequence around aa.187~191 (V-D-S-V-A) derived from Human MKK3 / MEK3. |
| 偶联标记 | Un-conjugated |
| 別名 | SAPK kinase 2; MEK 3; MAPKK 3; Stress-activated protein kinase kinase 2; PRKMK3; EC 2.7.12.2; MAPK/ERK kinase 3; MEK3; MAPKK3; SAPKK2; SAPKK-2; MAP kinase kinase 3; MKK3; Dual specificity mitogen-activated protein kinase kinase 3 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Antibodies were produced by immunizing rabbits with KLH-conjugated synthetic peptide. Antibodies were purified by affinity-chromatography using epitope-specific peptide. |
| 缓冲液 | PBS (without Mg2+ and Ca2+, pH 7.4), 150mM NaCl, 0.02% Sodium azide and 50% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # P46734 Human Dual specificity mitogen-activated protein kinase kinase 3 |
|---|---|
| 基因名称 | MAP2K3 |
| 全名 | mitogen-activated protein kinase kinase 3 |
| 背景介绍 | MEK3 belongs to MAPKK family. This kinase is activated by mitogenic and environmental stress, and participates in the MAPK-mediated signaling cascade. It phosphorylates and thus activates p38. This kinase can be activated by insulin, and is necessary for the expression of glucose transporter. Expression of Ras oncogene is found to result in the accumulation of the active form of this kinase, which thus leads to the constitutive activation of p38, and confers oncogenic transformation of primary cells. The inhibition of this kinase is involved in the pathogenesis of Yersina pseudotuberculosis. |
| 生物功能 | Dual specificity kinase. Is activated by cytokines and environmental stress in vivo. Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in the MAP kinase p38. [UniProt] |
| 研究领域 | Cancer antibody; Gene Regulation antibody; Immune System antibody; Signaling Transduction antibody |
| 预测分子量 | 39 kDa |
| 翻译后修饰 | Autophosphorylated. Phosphorylation on Ser-218 and Thr-222 by MAP kinase kinase kinases regulates positively the kinase activity (PubMed:8622669). Phosphorylated by TAOK2 (PubMed:11279118). Yersinia yopJ may acetylate Ser/Thr residues, preventing phosphorylation and activation, thus blocking the MAPK signaling pathway. |
检测图片 (3) Click the Picture to Zoom In
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ARG51108 anti-MEK3 / MKK3 antibody ICC/IF image
Immunofluorescence: Methanol-fixed HeLa cells stained with ARG51108 anti-MEK3 / MKK3 antibody.
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ARG51108 anti-MEK3 / MKK3 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human breast carcinoma tissue stained with ARG51108 anti-MEK3 / MKK3 antibody (left) or the same antibody preincubated with blocking peptide (right).
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ARG51108 anti-MEK3 / MKK3 antibody WB image
Western blot: Extracts from MDA cells stained with ARG51108 anti-MEK3 / MKK3 antibody.
