ARG41730
anti-MEFV / Pyrin antibody
anti-MEFV / Pyrin antibody for Western blot and Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes MEFV / Pyrin |
|---|---|
| 反应物种 | Ms, Rat |
| 应用 | WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | MEFV / Pyrin |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide corresponding to aa. 5-39 of Human MEFV / Pyrin. (PSDHLLSTLEELVPYDFEKFKFKLQNTSVQKEHSR) |
| 偶联标记 | Un-conjugated |
| 別名 | FMF; MEF; TRIM20; Pyrin; Marenostrin |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||
| 实际分子量 | ~ 86 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | 0.2% Na2HPO4, 0.9% NaCl, 0.05% Sodium azide and 5% BSA. |
| 抗菌剂 | 0.05% Sodium azide |
| 稳定剂 | 5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | MEFV |
| 全名 | Mediterranean fever |
| 背景介绍 | This gene encodes a protein, also known as pyrin or marenostrin, that is an important modulator of innate immunity. Mutations in this gene are associated with Mediterranean fever, a hereditary periodic fever syndrome. [provided by RefSeq, Jul 2008] |
| 生物功能 | Involved in innate immunity and the inflammatory response. Interacts with several components of the inflammasome complex, a large oligomeric structure which recruits and activates CASP1 and ultimately induces maturation of cytokines such as IL1B. However, the exact role of MEFV in the inflammatory pathway is uncertain as contradictory effects on IL1B processing have been reported in different experimental systems. Has been shown to activate IL1B production. Has also been shown to inhibit IL1B production. Also required for PSTPIP1-induced PYCARD oligomerization and for formation of pyroptosomes, large supramolecular structures composed of oligomerized PYCARD dimers which form prior to inflammatory apoptosis. Can reduce PYCARD-induced apoptosis. Recruits PSTPIP1 to pyroptosomes, and required for PSTPIP1 oligomerization. [UniProt] |
| 细胞定位 | Isoform 1: Cytoplasm, cytoskeleton, cytoplasmic vesicle, autophagosome. Cell projection, ruffle, lamellipodium. Nucleus. Note=Associated with microtubules and with the filamentous actin of perinuclear filaments and peripheral lamellar ruffles. In pre-apoptotic cells, colocalizes with PYCARD/ASC in large specks (inflammasomes). In migrating monocytes, strongly polarized at the leading edge of the cell where it colocalizes with polymerizing actin and PYCARD/ASC. Isoform 2: Nucleus. [UniProt] |
| 预测分子量 | 86 kDa |
| 翻译后修饰 | Cleaved by CASP1 (Probable). The N-terminal cleavage product localizes to the nucleus as a filamentous network and to the cytoplasm, interacts more strongly with RELA and NFKBIA than the full-length protein, enhances the nuclear localization of RELA and induces NFKBIA proteolysis. The C-terminal cleavage product localizes to the cytoplasm. [UniProt] |
检测图片 (1) Click the Picture to Zoom In
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ARG41730 anti-MEFV / Pyrin antibody WB image
Western blot: 50 µg of Rat spleen, 50 µg of Rat lung and 40 µg of HEPA whole cell lysates stained with ARG41730 anti-MEFV / Pyrin antibody at 0.5 µg/ml dilution.
