ARG45134
anti-MED8 antibody
anti-MED8 antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes MED8 |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | FACS, ICC/IF, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | MED8 |
| 抗原物种 | Human |
| 抗原 | Recombinant protein containing to human MED8. |
| 偶联标记 | Un-conjugated |
| 別名 | MED8; mediator complex subunit 8; Mediator of RNA polymerase II transcription subunit 8; Activator-recruited cofactor 32 kDa component; ARC32; Mediator complex subunit 8 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||||
| 实际分子量 | 29 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | 0.2% Na2HPO4, 0.9% NaCl, 0.05% Sodium azide and 4% Trehalose. |
| 抗菌剂 | 0.05% Sodium azide |
| 稳定剂 | 4% Trehalose |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q96G25 Human Mediator of RNA polymerase II transcription subunit 8 Swiss-port # Q9D7W5 Mouse Mediator of RNA polymerase II transcription subunit 8 |
|---|---|
| 基因名称 | MED8 |
| 全名 | mediator complex subunit 8 |
| 背景介绍 | This gene encodes a protein component of the mediator complex, which aids in transcriptional activation through interaction with RNA polymerase II and gene-specific transcription factors. The encoded protein may also function in ubiquitin ligation and protein degradation. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jan 2013] |
| 生物功能 | Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. May play a role as a target recruitment subunit in E3 ubiquitin-protein ligase complexes and thus in ubiquitination and subsequent proteasomal degradation of target proteins. [UniProt] |
| 细胞定位 | Nucleus. [UniProt] |
| 预测分子量 | 29 kDa |
| 翻译后修饰 | Phosphoprotein. [UniProt] |
检测图片 (7) Click the Picture to Zoom In
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ARG45134 anti-MED8 antibody WB image
Western blot: THP-1 and HepG2 stained with ARG45134 anti-MED8 antibody at 0.5 μg/ml dilution.
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ARG45134 anti-MED8 antibody FACS image
Flow Cytometry: 293T stained with ARG45134 anti-MED8 antibody at 1 µg/10^6 cells dilution.
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ARG45134 anti-MED8 antibody IHC-P image
Immunohistochemistry: Rat testis stained with ARG45134 anti-MED8 antibody at 1 μg/ml dilution.
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ARG45134 anti-MED8 antibody ICC/IF image
Immunofluorescence: U87 stained with ARG45134 anti-MED8 antibody at 5 ug/ml dilution.
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ARG45134 anti-MED8 antibody WB image
Western blot: Rat brain and rat liver stained with ARG45134 anti-MED8 antibody at 0.5 μg/ml dilution.
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ARG45134 anti-MED8 antibody IHC-P image
Immunohistochemistry: Mouse testis stained with ARG45134 anti-MED8 antibody at 1 μg/ml dilution.
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ARG45134 anti-MED8 antibody WB image
Western blot: Mouse brain and mouse liver stained with ARG45134 anti-MED8 antibody at 0.5 μg/ml dilution.
