ARG45114

anti-MED4 antibody

anti-MED4 antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat

概述

产品描述 Rabbit Polyclonal antibody recognizes MED4
反应物种 Hu, Ms, Rat
应用 FACS, ICC/IF, IHC-P, WB
宿主 Rabbit
克隆 Polyclonal
同位型 IgG
靶点名称 MED4
抗原物种 Human
抗原 E.coli-derived human MED4 recombinant protein
偶联标记 Un-conjugated
別名 MED4 ; Mediator Complex Subunit 4; DRIP36; HSPC126; TRAP36; VDRIP; Vitamin D3 Receptor-Interacting Protein Complex 36 KDa Component ; Mediator Of RNA Polymerase II Transcription Subunit 4; Activator-Recruited Cofactor 36 KDa Component; ARC36 ; Mediator Of RNA Polymerase II Transcription, Subunit 4 Homolog (S. Cerevisiae); Vitamin D Receptor-Interacting Protein, 36-KD; Vitamin D Receptor Interacting Protein; TRAP/SMCC/PC2 Subunit P36 Subunit; Mediator, 34-KD Subunit, Homolog

应用说明

应用建议
应用 推荐稀释比
FACS1-3 μg/10^6 cells
ICC/IF5 μg/ml
IHC-P2-5 μg/ml
WB0.1-0.25 μg/ml
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式 Liquid
纯化 Affinity purification with immunogen.
缓冲液 0.9% NaCl, 0.2% Na2HPO4 and 4% Trehalose
稳定剂 4% Trehalose
浓度 0.5 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 29079 Human MED4

GeneID: 306030 Rat MED4

GeneID: 67381 Mouse MED4

基因名称 MED4
全名 Mediator Complex Subunit 4
背景介绍 This gene encodes a component of the Mediator complex. The Mediator complex interacts with DNA-binding gene-specific transcription factors to modulate transcription by RNA polymerase II. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jul 2012]
生物功能 Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. [Unuprot]
细胞定位 Nucleus. [Unuprot]
预测分子量 30 kDa
翻译后修饰 Acetylation, Phosphoprotein. [Unuprot]

检测图片 (2) Click the Picture to Zoom In

  • ARG45114 anti-MED4 antibody ICC/IF image

    Immunofluorescence: HeLa stained with ARG45114 anti-MED4 antibody at 5 μg/ml dilution.

  • ARG45114 anti-MED4 antibody WB image

    Western blot: ANA-1 stained with ARG45114 anti-MED4 antibody at 0.25 μg/ml dilution.